Plastic-compatible high-resolution, large-FOV objectives
CFI S Plan Fluor LWD 20XC and CFI S Plan Fluor LWD ADM 20XC objectives for biological microscopes are designed for stem cell applications and drug design. With long working distances*1 and high numerical apertures, these objectives provide bright, high-resolution images through plastic cell-culture dishes.
The CFI S Plan Fluor LWD 20XC is suitable for brightfield, fluorescence and DIC observation, and the CFI S Plan Fluor LWD ADM 20XC can be used for brightfield, fluorescence and phase contrast observation.
Long working distances and high NA
The new objectives provide long working distances of 1.3 mm to 2.3 mm, which are compatible with imaging through thick-bottomed plastic dishes and observations of thick specimens. In addition, their high numerical aperture (0.70) enables collection of even dim fluorescent samples, resulting in clear, bright images with short exposure times.
25 mm FOV provides large viewing and image capture fields
The new objectives provide 20X observations with an FOV of 25 mm*2 when used together with the ECLIPSE Ti2 Inverted Research Microscope. The large field of view results in reduced acquisition times during high content screening and large image stitching*3, improving overall efficiency of workflows and increasing data throughput.
*1 Working distance: The distance from the tip of the objective lens to the observation target when the target is in focus. A long working distance has numerous advantages, such as prevention of contact with the sample when changing objectives.
*2 FOV (field of view: the diameter of the observable image in a microscope)
*3 Image stitching: an image processing method that combines neighboring images to create one large. Also known as image tiling.
|Cover glass thickness||Correction ring||Observation|
|CFI S Plan Fluor LWD 20XC||Diagram||graph||0.70||1.3-2.3||0-1.8||∨||BF, DF (Dry/Oil), DIC, POL, FL (visible light, UV)|
|CFI S Plan Fluor LWD ADM 20XC||Diagram||graph||0.70||1.3-2.3||0-1.8||∨||BF, DF (Dry/Oil), PH, FL (visible light, UV)|
PH: Phase contrast
POL: Simple polarizing
*Possible but not recommended
**External phase contrast observation is possible with Eclipse Ti2-E