Specifications

	AX	AX R
Scan Head	FN 25 galvano scanner Up to 8192 x 8192 pixels Up to 10 fps at 512 x 512 pixels Pixel dwell time up to 0.2 microseconds Supports bidirectional imaging and line-scan imaging	FN 25 resonant scanner FN 25 galvano scanner Up to 2048 x 2048 pixels for 2K (1024 x 1024 pixels for 1K) Up to 720 fps at 2048 x 16 pixels for 2K (720 fps at 1024 x 16 pixels for 1K) 30 fps at 2048 x 512 pixels for 2K (1024 x 512 pixels for 1K) Supports bidirectional imaging and line-scan imaging
Scan Head input/output port	One FC fiber laser input port and one FC fiber signal output port An additional FC fiber laser input port and FC fiber signal output port can be optionally added
Laser	Up to 16 wavelengths¹ of visible/near-IR lasers can be installed (when combined with the optional laser input port and AX-LU-EXP AX laser expansion unit) Compatible laser: (Standard) 405 nm to 750 nm, (option) 730 nm or 785 nm for NIR Imaging Option
Dichroic Mirror	Up to 6 customizable mirrors
DUX-VB Detector	2 or 4 channels Freely tunable emission bands with ± 1nm accuracy and up to 66 discrete spectral channels Up to 12 bandpass filters Multi-alkali PMT or GaAsP PMT options
DUX-ST Detector	2 or 4 channels Up to 18 bandpass filters Multi-alkali PMT or GaAsP PMT options
NSPARC Detector Unit	Lateral resolution 100 nm^1, Axial resolution 300 nm^1 Equipped with SPPC (Single Pixel Photon Counter) array detector Up to 7 barrier filters can be mounted, including multiple bandpass filters With galvano scanner: Can be used with X resolution of 64 to 8192 pixels, Y resolution of 2 to 8192 pixels With resonant scanner: Can be used with X resolution of 256, 512, 1024 and 2048 pixels, Y resolution of 128 to 2048 pixels
Diascopic Detector	Compact PMT detector
Pinhole	Continuously variable
Field Number	25
Z step	Ti2-E: 0.01 μm, 0.02 μm (with encoder control), FN1 stepping motor: 0.05 μm, Ni-E: 0.025 μm
Compatible microscopes	Ti2-E inverted microscope with maximum FOV of 25 mm Ni-E and FN1 upright microscopes with maximum FOV of 25 mm
Options	Photostimulation (point raster or digital micromirror) Fluorescence lifetime imaging including fast FLIM Piezoelectric Z (or XYZ) Environmental chamber or enclosure Other modalities such as TIRF, N-STORM
Software	Nikon NIS-Elements C Optional modules available Up to 16 bit images (65,536 gray levels with fine integration) with multiple file output options
Control Workstation	Microsoft Windows® 10 64bit/11 Professional with GPU-accelerated graphics card^*2
Recommended installation conditions	Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)

*1 These values were measured using 40 nm diameter beads for lateral resolution and 100 nm diameter beads for axial resolution with excitation by a 488 nm laser. Actual resolution depends on laser wavelength and optical configuration.

*2 Windows 10 and Windows 11 are trademarks or registered trademarks of Microsoft Corporation in the United States and other countries.