AX / AX R with NSPARC

Confocal Microscope System

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Specifications

AX AX R
Scan Head 25 mm FOV galvano scanner
Up to 8192 x 8192 pixels
Up to 10 fps at 512 x 512 pixels
Pixel dwell time up to 0.2 microseconds
Supports bidirectional imaging and line-scan imaging		 25 mm FOV resonant scanner
25 mm FOV galvano scanner
Up to 2048 x 2048 pixels for 2K (1024 x 1024 pixels for 1K)
Up to 720 fps at 2048 x 16 pixels for 2K (720 fps at 1024 x 16 pixels for 1K)
30 fps at 2048 x 512 pixels for 2K (1024 x 512 pixels for 1K)
Supports bidirectional imaging and line-scan imaging
Scan Head input/output port One FC fiber laser input port and one FC fiber signal output port
An additional FC fiber laser input port and FC fiber signal output port can be optionally added
Laser Up to 8 visible/near-IR lasers
Compatible laser: (Standard) 405 nm to 750 nm, (option) 730 nm or 785 nm for NIR Imaging Option
Dichroic Mirror Up to 6 customizable mirrors
DUX-VB Detector 2 or 4 channels
Freely tunable emission bands with ± 1nm accuracy and up to 66 discrete spectral channels
Up to 12 bandpass filters
Multi-alkali PMT or GaAsP PMT options
DUX-ST Detector 2 or 4 channels
Up to 18 bandpass filters
Multi-alkali PMT or GaAsP PMT options
NSPARC Detector Unit Lateral resolution 100 nm*1, Axial resolution 300 nm*1
Equipped with SPPC (Single Pixel Photon Counter) array detector
Up to 7 barrier filters can be mounted (supports excitation at 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm and 640 nm)
With galvano scanner: Can be used with X resolution of 64 to 8192 pixels, Y resolution of 2 to 8192 pixels
With resonant scanner: Can be used with X resolution of 256, 512 and 1024 pixels, Y resolution of 128 to 1024 pixels*2
Diascopic Detector Compact PMT detector
Pinhole

Continuously variable
FOV Maximum 25 mm diameter (circle) inscribed by a rectangle
Z step Ti2-E: 0.01 μm, 0.02 μm (with encoder control), FN1 stepping motor: 0.05 μm, Ni-E: 0.025 μm
Compatible microscopes Ti2-E inverted microscope with maximum FOV of 25 mm
Ni-E and FN1 upright microscopes with maximum FOV of 25 mm
Options Photostimulation (point raster or digital micromirror)
Fluorescence lifetime imaging including fast FLIM
Piezoelectric Z (or XYZ)
Environmental chamber or enclosure
Other modalities such as TIRF, N-STORM or N-SIM S
Software Nikon NIS-Elements C
Optional modules available
Up to 16 bit images (65,536 gray levels with fine integration) with multiple file output options
Control Workstation

Microsoft Windows® 10 64bit/11 Professional with GPU-accelerated graphics card
Recommended installation conditions Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)

*1 These values were measured using 40 nm diameter beads for lateral resolution and 100 nm diameter beads for axial resolution with excitation by a 488 nm laser. Actual resolution depends on laser wavelength and optical configuration.
*2 Resolution of 2048 pixels cannot be set.