Nikon’s Confocal NIS-Elements Package
Dedicated interface for Nikon’s confocal and multiphoton systems, providing easy instrument setup and streamlined operation. Incorporates many of the features of NIS-Elements AR for advanced acquisition, image processing, analysis, visualization and data sharing capability.
Key Features
Resolution
Image resolution is defined as the smallest distance between 2 points that can be resolved. The theoretical limit of resolution for a conventional optical microscope is approx. 200 nm. Higher resolution images can be theoretically achieved with confocal microscopes, but this has not been effectively achieved. Resolution can be increased beyond the conventional confocal image resolution (up to ~1.5 times improvement in XY; up to ~1.7 times improvement in Z) by using specific image acquisition and processing techniques.
Standard confocal image
Enhanced Resolution image
Zebrafish lens at 5 dpf. Nuclei (green) and actin filaments (red) are visualized with Cytox-green and Rhadamine-conjugated phalloidin, respectively. High magnification images indicate lens fiber cells, which become flat and stack against each other.
Image courtesy of: Drs. Toshiaki Mochizuki and Ichiro Masai, Developmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University
Standard confocal image
Enhanced Resolution image
Red: Central spindle, Blue: Nuclei
Image courtesy of: Toshinori Hyodo Ph.D., Department of Biochemistry, Aichi Medical University School of Medicine
Standard confocal image
Enhanced Resolution image
Stress Fibers (LLC-PK1, Pig Kidney Cells), Green: F-actin, Red: Myosin Heavy Chain
Image courtesy of: Keiju Kamijo Ph.D., Division of Anatomy and Cell Biology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University
Standard confocal image
Enhanced Resolution image
Apical surfaces of auditory epithelia of mouse cochleae were stained by Atto-565-phalloidin at postnatal day 2.
Image courtesy of: Dr. Hideru Togashi, Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine
See for yourself…test our deconvolution for free!
NIS-Elements offers advanced 3D and 2D deconvolution modules for improving image quality. Upload your image to our NIS-Elements deconvolution test site to see the difference.
Product Brochure
Software
- NIS-Elements
