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Confocal Microscope System

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Superior Optics for Confocal

Controlling the manufacturing and implementation of optics from raw materials all the way to complete microscope systems brings unparalleled optical quality and performance.

Complementary optical design means the confocal system, microscope, and objectives all are optimized and matched for superior quality and resolution.

Nikon’s CFI60 and 75 infinity corrected optical system has numerous options for magnifications, working distances, and immersion mediums, paired for use with an extremely wide variety of samples and specimen preparations.

Nano Crystal Coat for superior transmission

Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that lowers the reflectance index, facilitating the passage of light through the lens rather than reflecting it, and thus providing superior light transmission.

CFI Plan Apochromat Lambda S 25XC Sil & 40XC Sil

This objective provides a high NA, large FOV and long working distance, and uses silicone oil with a refractive index approximating that of live cells as the immersion liquid. It enables clear observation even in the deeper regions of thick samples.

Working distance: 0.55mm / 0.30mm
Numerical aperture:

1.05 / 1.25
Chromatic aberration correction: from visible to UV
Nano Crystal Coat applied✔︎

CFI Plan Apochromat LWD Lambda S 20XC WI & 40XC WI

This objective corrects chromatic aberrations in a wide wavelength range from visible to near-IR light. With its high numerical aperture, as well as long working distance and high image flatness, it is a powerful tool for imaging thick living specimens.

Working distance: 0.93mm / 0.63mm
Numerical aperture: 0.95 / 1.15
Chromatic aberration correction: from visible to infrared
Nano Crystal Coat applied✔︎

CFI Apochromat LWD Lambda S 20XC WI & 40XC WI

This highly versatile objective provides superior performance thanks to its high numerical aperture, wide field of view and long working distance.

Working distance:

0.95mm / 0.6mm
Numerical aperture:

0.95 / 1.15
Chromatic aberration correction: from UV through to near IR
Nano Crystal Coat applied✔︎

CFI Apochromat Lambda D 10X

This objective provides superb aberration correction up to the periphery of its large 25 mm field of view, enabling sharp image acquisition of entire samples with digital cameras that have large image sensors. It also corrects chromatic aberration over a wide wavelength range.

CFI Apochromat VC 60XC WI

A water immersion objective that ensures clear image acquisition even within the deep areas of samples. It corrects chromatic aberrations in the shorter wavelength range and is suitable for multicolor confocal imaging.

Objectives for confocal microscopy

Model NA W.D (mm) Nano Crystal Coat
CFI Plan Apochromat Lambda D 2X 0.1 8.5 ○
CFI Plan Apochromat Lambda D 4X 0.2 20.0 ○
CFI Plan Apochromat Lambda D 10X 0.45 4.0 ○
CFI Plan Apochromat Lambda D 20X 0.8 0.8 ○
CFI Plan Apochromat Lambda D 40X 0.95 0.21 ○
CFI Plan Apochromat Lambda D 60X Oil 1.42 0.15 ○
CFI Plan Apochromat Lambda D 100X Oil 1.45 0.13 ○
CFI Plan Apochromat Lambda S 25XC Sil 1.05 0.55 ○
CFI Plan Apochromat Lambda S 40XC Sil 1.25 0.30 ○
CFI Plan Apochromat VC 60XC WI 1.20 0.29 -
CFI Plan Apochromat 10XC Glyc*1 0.50 5.50 ○
CFI Plan Apochromat Lambda 60XC 0.95 0.15 ○
CFI Plan Apochromat IR 60XC WI 1.27 0.17 ○
CFI SR HP Plan Apochromat Lambda S 100XC Sil 1.35 0.30 (0.31-0.29): 23˚C,
0.29 (0.30-0.28): 37˚C		 ○
CFI Apochromat LWD Lambda S 20XC WI 0.95 0.95 ○
CFI Apochromat LWD Lambda S 40XC WI 1.15 0.6 ○
CFI Plan Apochromat LWD Lambda S 20XC WI 0.95 0.93 ○
CFI Plan Apochromat LWD Lambda S 40XC WI 1.15 0.63 ○

*1: If you use a tissue clearing reagent, instead of glycerin, as an immersion liquid, some tissue clearing reagents may damage these products. Please confirm with a distributor before purchasing the product.