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Confocal Microscope System

Superior Optics for Confocal

Controlling the manufacturing and implementation of optics from raw materials all the way to complete microscope systems brings unparalleled optical quality and performance.

Complementary optical design means the confocal system, microscope, and objectives all are optimized and matched for superior quality and resolution.

Nikon’s CFI60 and 75 infinity corrected optical system has numerous options for magnifications, working distances, and immersion mediums, paired for use with an extremely wide variety of samples and specimen preparations.


Nano Crystal Coat for superior transmission

Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that lowers the reflectance index, facilitating the passage of light through the lens rather than reflecting it, and thus providing superior light transmission.

CFI Plan Apochromat Lambda S 40XC Sil

This objective provides bright, high-resolution images, and is suitable for imaging of thick samples and long-term time-lapse imaging.

Working distance: 0.30mm
Numerical aperture: 1.25
Chromatic aberration correction: from visible to UV
Nano Crystal Coat applied✔︎

CFI Plan Apochromat Lambda S 25XC Sil

This objective provides a high NA, large FOV and long working distance, and uses silicone oil with a refractive index approximating that of live cells as the immersion liquid. It enables clear observation even in the deeper regions of thick samples.

Working distance: 0.55mm
Numerical aperture: 1.05
Chromatic aberration correction: from visible to UV
Nano Crystal Coat applied✔︎

CFI Plan Apochromat Lambda S 10X

This objective supports image acquisition with digital cameras having large image sensors, by improving aberration correction up to the periphery of a large FOV of 25 mm, and is suitable for confocal imaging.

Working distance: 4mm
Numerical aperture: 0.45
Chromatic aberration correction: from visible to UV
Nano Crystal Coat applied✔︎

CFI Apochromat LWD Lambda S 20XC WI

This highly versatile objective provides superior performance thanks to its high numerical aperture, wide field of view and long working distance.

Working distance: 0.95mm
Numerical aperture: 0.95
Chromatic aberration correction: from visible to infrared
Nano Crystal Coat applied✔︎

CFI Apochromat LWD Lambda S 40XC WI

Its high NA for water immersion objectives provides brighter and higher-resolution images and makes this lens ideal for confocal live cell imaging.

Working distance: 0.6mm
Numerical aperture: 1.15
Chromatic aberration correction: from UV through to near IR
Nano Crystal Coat applied✔︎

Objectives for confocal microscopy

model NA W.D (mm) Nano Crystal Coat
CFI Plan Apochromat Lambda S 10X 0.45 4.00
CFI Plan Apochromat VC 20X 0.75 1.00 -
CFI Plan Apochromat Lambda S 25XC Sil 1.05 0.55
CFI Plan Apochromat Lambda S 40XC Sil 1.25 0.30
CFI Plan Apochromat VC 60XC WI 1.20 0.29 -
CFI Plan Apochromat 10XC Glyc 0.50 5.50
CFI Plan Apochromat Lambda 20X 0.75 1.00
CFI Plan Apochromat Lambda 40XC 0.95 0.21
CFI Plan Apochromat Lambda 60XC 0.95 0.15
CFI Plan Apochromat Lambda 60X Oil 1.40 0.13
CFI Plan Apochromat IR 60XC WI 1.27 0.17
CFI Plan Apochromat VC 100X Oil 1.40 0.13 -
CFI Plan Apochromat Lambda 100X Oil 1.45 0.13
CFI SR HP Plan Apochromat Lambda S 100XC Sil 0.30 1.35
CFI Apochromat LWD Lambda S 20XC WI 0.95 0.95
CFI Apochromat LWD Lambda S 40XC WI 1.15 0.60