Confocal and Multiphoton Microscopes
Confocal microscopy provides optical sectioning, the ability to observe discrete planes in 3D samples, by using one or more apertures to block out-of-focus light. Nikon offers both point-scanning confocal instruments, led by our AX / AX R confocal system, as well as spinning disk (field scanning) confocal systems from top manufacturers.
Multiphoton microscopy is preferred for deep imaging applications in thick specimens, including intravital imaging. Non-linear excitation restricts fluorescence to the laser focus and near-infrared illumination minimizes absorption and scattering. Nikon offers the AX R MP multiphoton system, available with microscope stand options optimized for large specimens.
Image scanning microscopy (ISM) is a super-resolution technique that takes advantage of structured detection of each point in a point-scanning system to improve both resolution and signal-to-noise (S/N), a great choice for low light imaging. Both the AX / AX R confocal and AX R MP multiphoton systems may be equipped with the Nikon Spatial Array Confocal (NSPARC) detector for ISM imaging.
AX/AX R
Widefield, super-resolution confocal laser scanning microscope
— the core of the AX series —
AX R MP with NSPARC
Achieves super-resolution and deep imaging while realizing low-noise images in deep observation via the NSPARC detector.
- Widefield
- High speed
- High resolution
- Deep imaging
- Long wavelength
- Analysis
- Minimally invasive
- Low phototoxicity
- In Vivo
AX R MP
Provides a wide field of view and fast imaging that are ideal for clear visualization of deep microstructures.
- Widefield
- Long wavelength
- High speed
- Deep imaging
- Analysis
- Minimally invasive
- Low phototoxicity
- In Vivo
AX / AX R with NSPARC
Equipped with an NSPARC detector for super-resolution, achieving exceptionally low-noise and high-spatial-resolution imaging.
- Widefield
- High speed
- High resolution
- Analysis
- Minimally invasive
- Low phototoxicity
- Multicolor
AX NIR
Realizes high wavelength selectivity with near-infrared excitation, making it ideal for live-cell long-wavelength imaging.
- Widefield
- High speed
- Analysis
- Minimally invasive
- Low phototoxicity
- Multicolor+NIR
- In Vivo
Product Lineup
New
AX / AX R with NSPARC
Nikon’s latest confocal microscope, leveraging deep learning-based AI tools to simplify acquisition and improve image quality. Fast scanning combined with a large 25 mm field of view enables high throughput imaging with unmatched edge-to-edge image quality. Super-resolution provided by the NSPARC detector.
New
AX R MP with NSPARC
The newest multiphoton microscope from Nikon is a top choice for deep imaging. The next generation 2K resonant scanner captures fast in vivo dynamics with high resolution over a large field of view. The AX R MP also supports confocal imaging with visible wavelengths and super-resolution using the NSPARC detector.
Yokogawa CSU Series
Spinning disk confocal systems from Yokogawa Life Sciences provide fast and user-friendly options for live cell, organoid, and other imaging applications. The CSU-W1 SoRa spinning disk system provides super-resolution using Optical Pixel Reassignment (OPR) technology.
CrestOptics X-Light Series
Spinning disk systems from CrestOptics are flexible and budget-friendly choices for a variety of applications. The large 25 mm field of view is a great match for high throughput imaging. The optional DeepSIM module provides super-resolution via multi-spot structured illumination.