Application Notes

3D Angiogenesis Assay Utilizing Microphysiological Systems

May 2025

Angiogenesis is associated with physiological processes such as reproduction, development, and wound healing. Simultaneously, it is intricately linked to diseases including cancer, autoimmune disorders, age-related macular degeneration, and atherosclerosis. Particularly in cancer, angiogenesis is a key factor behind tumor growth and metastasis, making it a hallmark of cancer and a vital target for drug discovery efforts. In vitro assays mimicking the angiogenesis process have been utilized for anti-cancer drug screening and other pharmaceutical developments. This application note introduces a validation study of a 3D angiogenesis assay using human umbilical vein endothelial cells (HUVECs) in AIM Biotech’s idenTx series Microphysiological Systems (MPS).