Digital Sight 100

A Wide Field of View and High Image Quality — Support for High-Precision, Comfortable Capturing

The Digital Sight 100 is a color digital camera for microscopes combining wide-field capture (Field Number 25), 17.7-megapixel resolution, and AdobeRGB 12-bit color support. Powered by Nikon's EXPEED 7 image processing engine, it delivers accurate color reproduction and low-noise imaging across brightfield and fluorescence applications.

Comprehensive connectivity — USB-C with power delivery, HDMI 4K output, and exclusive Wi-Fi capability — makes the Digital Sight 100 adaptable to PC-based, tablet-based, and monitor-direct workflows. The successor to the DS-Fi3 offers a significantly higher resolution, a wider field of view, and modern interfaces.

CMOS Sensor	Field of View	Full-HD Live	Color Depth	Pixel Size
17.7MP	FN25	60fps	12bit	2.4µm

The Digital Sight 100 is not for clinical diagnostic use. Specifications are subject to change without notice.

Nikon’s Photography Heritage, Brought to the Microscope

The Digital Sight 100 incorporates the same EXPEED 7 image-processing engine that powers Nikon's acclaimed mirrorless cameras like the Z9, enabling high-speed processing of high-resolution data generated by the camera. In microscopy, EXPEED 7 delivers faster frame rates, enables simultaneous image output to both an external monitor and a PC or tablet, and produces faithful color rendering across the full 12-bit pipeline. The result is images where stain differentiation is sharp, fluorescent signals emerge cleanly from background, and fine structural detail is preserved with every acquisition — automatically, without post-processing.

ADVANCED NOISE REDUCTION
FAITHFUL COLOR RENDERING
WIDE DYNAMIC RANGE
REAL-TIME 12-BIT PIPELINE
SIMULTANEOUS MONITOR + PC OUTPUT

Brochure

Download Digital Camera Brochure (4.9MB)

Key Features

Wide-field Capture

Large FOV Imaging — Field Number 25

Wide-field photography with a Field Number of 25 enables the observation and capture of a large area in a single shot. Live sharing during monitor observation and capturing images provide a field of view equivalent to binocular vision, enhancing work efficiency.

The Digital Sight 100 captures an area approximately 3x larger than conventional Field Number 16 cameras at the same magnification. Alternatively, step up to a higher NA objective for improved resolution while maintaining equivalent coverage to previous-generation cameras.

Field Number	More Area vs FN16	ASPECT MODES
25	~3x	4:3 & 16:9

Single-shot capture of large tissue sections — H&E, Trichrome, IHC
Binocular-equivalent field of view for live observation
Reduces stitching requirements for large area imaging
Compatible with Nikon ECLIPSE Ni and Ti2 platforms

Color Reproduction

High-Level Color Reproduction — sRGB & Adobe RGB

AdobeRGB (Digital Sight 100) covers a significantly wider color gamut than sRGB, enabling more faithful reproduction of subtle stain differences.

In addition to the conventional sRGB color space, the adoption of Adobe RGB with its wider color gamut has extended the color reproduction capabilities. The expanded color gamut effectively enhances color reproduction for scientific imaging applications.*

Full 12-bit color depth provides 4,096 levels of tonal information per channel — preserving the subtle intensity differences critical to histochemical stain differentiation and quantitative fluorescence analysis. EXPEED 7 ensures this color accuracy is maintained in real-time during live viewing.

Color Depth	sRGB + ADOBE RGB
12bit	2 Modes

sRGB and Adobe RGB color space, selectable per workflow
12-bit output — 4,096 tonal levels per channel
ICC profile compatibility for calibrated display workflows
Accurate rendering of common histological and IHC stains

* a display supporting the extended color gamut of Adobe RGB is required for extended gamut visualization, but not for analysis of acquired images

Fluorescence Imaging

High-Resolution Fluorescence Capturing

HeLa cells Nucleus (DAPI) , Tubulin (FITC), Mitochondria(Mito Tracker RedFM), Acquired using a Nikon standard sample.
Multi-channel fluorescence image. The 1-inch CMOS sensor collects more photons per frame, improving signal-to-noise in low-light fluorescence conditions.

The adoption of a large 1-inch CMOS sensor allows for highly sensitive capturing of images, which enhances convenience during fluorescence observation. The increased sensor area collects more photons per frame, translating directly into improved signal-to-noise ratios for weak fluorescent signals.

EXPEED 7 processing applies real-time noise reduction without compromising signal integrity, enabling confident co-localization analysis and subcellular structure visualization across multiple fluorescence channels.

Large 1-inch sensor maximizes fluorescence signal collection
EXPEED 7 real-time noise reduction preserves signal fidelity
Multi-channel compatibility: DAPI, FITC, TRITC and others
12-bit fluorescence output for quantitative intensity analysis
Exposure range 100 µs–10 s for flexible illumination conditions

Remote Workflow

Remote Capturing via Wi-Fi

Two Wi-Fi connection modes: Station Mode for live streaming via a network router, and Access Point Mode for direct stable connections with multiple users.

A PC with free NIS-Elements LE software installed can be connected via Wi-Fi to capture microscope images. The Digital Sight 100 enables untethered live image streaming, remote capture, and instant data sharing without a tethered USB connection.

Two Wi-Fi connection modes are available: Station Mode for institutional network integration (conferences, lectures) and Access Point Mode for direct multi-user connections from tablets and smartphones without a router.

modes
2

Station Mode: router-connected for conferences and lectures
Access Point Mode: direct multi-device connection, no router required
JPEG image transfer over Wi-Fi connection

Connectivity

HDMI Output & USB-C Power Delivery

Digital Sight 100 — USB-C (data + power), HDMI 4K output, and USB-A host port for Wi-Fi/Ethernet dongle

An HDMI terminal is provided to allow users to connect directly to a display and view 4K images without a personal computer. PC-free observation makes the Digital Sight 100 ideal for bench setups, conference rooms, and demonstration environments.

The USB-C connector (USB 3.2 Gen 2) handles both high-speed data transfer and power delivery simultaneously — a single cable from your PC powers the camera and transfers images at up to 10 Gbps, eliminating the need for a separate AC power adapter in most setups.

DATA + POWER	4K PC-FREE	SIMULTANEOUS OUTPUT
USB-C	HDMI	USB+HDMI

USB-C (USB 3.2 Gen 2): high-speed data + power delivery
USB-A (USB 3.2 Gen 1): host port for Wi-Fi or Ethernet dongle
HDMI 1.4b: 4K direct display output, no PC required
Simultaneous output to monitor and PC/tablet via EXPEED 7

Image Gallery

See the Difference in Every Image

Representative brightfield images acquired with the Digital Sight 100. AdobeRGB 12-bit color faithfully captures the subtle hue differences between staining protocols.

System Integration

Connect to Any Workflow

The Digital Sight 100 integrates into PC-based, tablet-based, and monitor-direct setups. Multiple interface options support dedicated research stations and flexible teaching or clinical environments.

Desktop PC

Windows 11 Pro (64-bit) with NIS-Elements LE (free) or NIS-Elements v7 for full imaging and analysis

Windows Tablet

Wi-Fi Exclusive to Digital Sight 100

Portable operation via NIS-Elements LE; combine with Wi-Fi for fully untethered, mobile imaging

4K HDMI Monitor

PC-free live 4K observation and image capture. Direct HDMI connection — no workstation needed at the bench

Network / Wi-Fi

Share live images wirelessly for teaching and consultation. Station Mode or Access Point Mode available

Applications

Built for Diverse Environments

From research and pathology to classroom teaching — the Digital Sight 100 adapts to your context with the same high-quality imaging performance.

Research & Life Sciences

High-fidelity color and fluorescence imaging for demanding scientific applications.

Cell culture documentation and monitoring
Histology and pathology overview imaging
Brightfield and multi-channel fluorescence
IHC / immunofluorescence staining analysis
Time-point documentation studies

Clinical Research & Pathology

Wide-field coverage and accurate stain color reproduction for clinical research documentation.

Tissue section overview (H&E, Trichrome, PAS)
Cytology and hematology screening support
Gout crystal documentation (polarized light)
Remote consultation image sharing via Wi-Fi
Audit-ready image archiving (TIFF, ND2)

Education & Training

Designed for group learning, demonstrations, and distributed digital access to live images.

Classroom live display via HDMI or Wi-Fi
Simultaneous multi-user remote viewing
PC-free demonstrations with HDMI monitor
Tablet-based operation for student stations
NIS-Elements LE: simple interface for new users

Imaging Software

NIS-Elements — Free Software Included

The Digital Sight 100 is fully compatible with Nikon's NIS-Elements software ecosystem. NIS-Elements LE is included at no cost, providing live display, camera control, image capture, scale bar overlay, annotation, and Wi-Fi support.

As imaging needs grow, NIS-Elements D, BR, and AR add progressively more powerful analysis capabilities, all sharing the same interface and file format for seamless workflow continuity.

Software Packages

NIS-Elements LE

Included — Free

Live display, camera control, image capture, scale bar, annotation, TIFF/JPEG/ND2 export, Wi-Fi support. For desktop and compatible tablet PCs.

NIS-Elements D (Documentation)

Optional

Adds extended depth of focus (EDF), large image stitching, calibrated manual measurements, report generation, and database integration.

NIS-Elements BR (Basic Research)

Optional

Adds 4D multidimensional imaging, multichannel fluorescence acquisition, Z-series capture, motorized microscope control, and expanded image analysis functions.

NIS-Elements AR (Advanced Research)

Advanced

Full 6D multidimensional imaging, motorized microscope control, Z-series 3D rendering, multichannel acquisition, and Segment.ai deep learning segmentation.