Nikon Imaging Center
Harvard Medical School
The Nikon Imaging Center at Harvard Medical School (NIC@HMS) is a Cell Biology Department, Systems Biology Department, and Biochemistry & Molecular Pharmacology Department core facility for light microscopy developed in partnership with Nikon Instruments Inc. and Micro Video Instruments Inc. The NIC@HMS also has several corporate contributors including Binder GmbH, CellASIC, Chroma Technology, CYTOO, Hamamatsu Photonics, Lumencor, Molecular Devices, Prior Scientific, Sutter Instrument Company and Tokai Hit Co., Ltd.
The mission of the NIC@HMS is to:
- Enhance basic research by providing access to state-of-the-art microscopy and imaging equipment
- Provide training courses on basic and advanced light microscopy techniques for the benefit of HMS and the greater Boston research community
- Introduce the latest innovations in light microscopy and imaging to the HMS community
- Serve as a learning center for our corporate partners and contributors
- Provide a dedicated NIC@HMS director for ongoing technical consultation and support
The NIC@HMS was the first Nikon Imaging Center, established in 2001. Nikon has since established several other Imaging Centers in collaboration with universities and research centers worldwide.
Contact
NIC Director
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Address
Harvard Medical School25 Shattuck St
Boston
MA 02115
United States
Website
Systems Available
CSU-X1 Spinning Disk Confocal System
George features a Ti inverted microscope with spinning disk confocal scanner and incubation, great for live cell imaging.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
Yokogawa CSU-X1 spinning disk confocal
- 5-line laser unit (445nm, 488nm, 515nm, 568nm, 642nm)
- Hamamatsu ORCA-Flash4.0 V3 sCMOS camera
- Lumencor SOLA light engine
- OkoLab enclosure incubation system
FRAP/CSU-X1 Spinning Disk Confocal System
Lucille features a spinning disk confocal scanner as well as FRAP device for live cell photoactivation/FRAP experiments.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
Yokogawa CSU-X1 spinning disk confocal
- 5-line laser unit (445nm, 488nm, 515nm, 568nm, 642nm)
- Hamamatsu ORCA-ER CCD camera
- Hamamatsu ORCA-R2 CCD camera
- Photonic Instruments MicroPoint laser FRAP/photoactivation unit
- Lumencor SOLA light engine
- Okolab stage-top incubation system
N-STORM/TIRF Super-Resolution System
Tobias is a single molecule imaging system featuring N-STORM for 3D single molecule localization and TIRF.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
N-STORM super-resolution single molecule localization microscopy system
- Agilent MLC400B 4-line laser unit (405nm, 488nm, 561nm, 647nm)
- Andor iXon 897 EMCCD camera
- Hamamatsu ImagEM EMCCD camera
- Lumencor SOLA light engine
NIS-Elements software
Large FOV Slide-Scanning System
Michael is a high-throughput large field of view system configured on a Nikon Ti2 and optimized for processing slides.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Large format DS-Qi2 monochrome CMOS camera
- Lumencor SPECTRA X light engine
NIS-Elements software
Microinjection System
Annyong is a Nikon TE300 inverted microscope configured for routine imaging applications.
Components
- TE2000-U inverted microscope
- Sutter XenoWorks Digital Microinjector
- Hamamatsu ORCA-ER CCD camera
- Lumencor SOLA light engine
Live-Cell Inverted Widefield System
George Michael is a short-term live-cell imaging system optimized for fast triggered acquisitions.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- Hamamatsu ORCA-R2 CCD camera
- Lumencor SPECTRA X light engine
- Tokai Hit stage-top incubation system
NIS-Elements software
Live-Cell Inverted Widefield System
Buster features a Ti2 inverted microscope with full incubation enclosure for long-term live-cell imaging.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- Lumencor SPECTRA X light engine
- OkoLab enclosure incubation system
NIS-Elements software
CSU-X1 Spinning Disk Confocal System
Lucille Two is another spinning disk confocal system for live cell imaging.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
Yokogawa CSU-X1 spinning disk confocal
- 5-line laser unit (445nm, 488nm, 515nm, 568nm, 642nm)
- Lumencor SOLA light engine
- OkoLab enclosure incubation system
Live-Cell Inverted Widefield System
Gob features a full enclosure type incubation system on an inverted microscope for long-term live-cell imaging.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
- Hamamatsu ORCA-ER CCD camera
- Lumencor SOLA light engine
- OkoLab enclosure incubation system
Inverted Widefield Microscope System
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Hamamatsu ORCA-Flash4.0 LT sCMOS camera
- DS-Fi1c color CCD camera
- Lumencor SOLA light engine
NIS-Elements software
A1Rsi Resonant Scanning Confocal System
Maeby is a fast live imaging system featuring an A1Rsi resonant scanning confocal system with spectral detector and FRAP/photoactivation.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
- A1Rsi resonant scanning confocal system
- 6-line laser unit (405nm, 457nm, 488nm, 514nm, 561nm, 640nm)
- A1-DUS 32-channel spectral detector
- Lumencor SOLA light engine
- Tokai Hit stage-top incubation system
NIS-Elements software
Live-Cell TIRF System
Lindsay is a TIRF imaging system optimized for fast live cell imaging.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
- Motorized TIRF Illuminator Unit
- 6-line laser unit (404nm, 446nm, 491nm, 515nm, 561nm, 642nm)
- Hamamatsu ImagEM EMCCD camera
- OkoLab enclosure incubation system
NIS-Elements software