Center of Excellence
Lunenfeld-Tanenbaum Research Institute
Located in the Lunenfeld-Tanenbaum Research Institute of Sinai Health, Canada’s first Nikon Centre of Excellence seeks to provide access to the latest in imaging technologies to its trainees, visiting scholars, and collaborators at the University of Toronto, the greater Toronto community and around the world. Our goal is to harness the power of advanced imaging and image analysis to address the most salient questions in cell biology and to foster meaningful relationships between our researchers and Nikon’s hardware and software development teams.
Contact
Director
Laurence Pelletier, Ph.D.
Address
Lunenfeld-Tanenbaum Research InstituteSinai Health
600 University Avenue Room 1070A
Toronto, ON Canada M5G 1X5
Website
Systems Available
A1R MP Resonant Scanning Multiphoton System
This system features the A1R MP resonant scanning multiphoton system with visible light confocal capabilities, based on the Ti2-E inverted microscope with stage-top incubation chamber, for a variety of live and fixed specimen imaging applications.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
- A1R MP resonant scanning multiphoton system
- Coherent Chameleon Discovery dual beam laser unit (680-1300nm)
- Episcopic GaAsP non-descanned detectors (NDDs)
- DU4G GaAsP 4-channel confocal detector
- Lumencor SOLA light engine
- Tokai Hit stage-top incubation system
NIS-Elements high content image acquisition and analysis software
A1R-HD25 Large Field of View Confocal and CREST X-Light V2 Large Field of View Spinning Disk System
The A1R-HD25 large field of view resonant scanning confocal system and CREST X-LIGHT V2 large field of view spinning disk confocal system are both configured on a Ti2-E inverted microscope, equipped with the stage-top incubation chamber for live cell imaging.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
A1R HD25 high-definition resonant scanning confocal system
LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- DU4G GaAsP 4-channel confocal detector
- Crest X-Light V2 L-FOV spinning disk confocal system
- Lumencor Celesta 7-line laser unit (405nm, 446nm, 477nm, 520nm, 546nm, 638nm, 749nm)
- Photometrics Prime95B 25mm sCMOS camera
- Lumencor SOLA light engine
- Tokai Hit stage-top incubation system
NIS-Elements high content image acquisition and analysis software
BioPipeline Live High Content Imaging System with A1R-HD25 Resonant Scanning Confocal
The A1R-HD25 large field of view resonant scanning confocal system on a Ti2-E inverted microscope base, equipped with the full enclosure incubation and robotic delivery system for multiwell plates, to enable long-term high content imaging of live samples.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
A1R HD25 high-definition resonant scanning confocal system
LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- DU4G GaAsP 4-channel confocal detector
- Lumencor Spectra III 8-line light engine
- 44 multiwell plate capacity incubator
- Full enclosure incubation system for imaging
- Robotic system for automated delivery of the plates from the incubator to the microscope stage
NIS-Elements high content image acquisition and analysis software
CSU-W1 SoRa Spinning Disk Confocal and Superresolution System
The CSU-W1 SoRa spinning disk with Cairn Research TwinCam optics for simultaneous dual camera imaging, configured on a Ti2-E inverted microscope base, combines confocal and super-resolution capabilities with wide variety of laser lines for fast and gentle multiwavelength imaging.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Yokogawa CSU-W1 SoRa spinning disk confocal
- LUN-F XL 7-line laser unit (405nm, 440nm, 488nm, 514nm, 561nm, 594nm, 640nm)
- Cairn Research TwinCam optics for simultaneous dual camera imaging
- Two Hamamatsu FusionBT sCMOS cameras
- Mad City Labs Nano-Z series Z-axis piezo stage
- Lumencor SOLA light engine
- Tokai Hit stage-top incubation system
NIS-Elements high content image acquisition and analysis software
CSU-X1 Spinning Disk Confocal System
The CSU-X1 spinning disk confocal system with W-View Gemini emission splitting optics, configured on a Ti2-E inverted microscope base, enables fast and gentle imaging of live and fixed specimens.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Yokogawa CSU-X1 spinning disk confocal
- Toptica Photonics iChrome MLE 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Andor Zyla 4.2 sCMOS camera
- Hamamatsu W-View Gemini image splitting optics
- Mad City Labs Nano-Z series Z-axis piezo stage
- Lumencor SOLA light engine
NIS-Elements high content image acquisition and analysis software
A1R-HD Resonant Scanning Confocal System with High-Speed Widefield Imaging Capabilities
The Nikon A1R-HD resonant scanning confocal system with spectral detection capabilities and equipment for high-speed widefield epifluorescence imaging is integrated on a Ti2-E inverted microscope base, for fast multichannel image acquisition.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
A1R HD high-definition resonant scanning confocal system
LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- DU4G GaAsP 4-channel confocal detector
- DUV-B 2-channel spectral GaAsP detector
- Hamamatsu FusionBT sCMOS camera
- Lumencor Spectra III 8-line light engine
- Mad City Labs Nano-Z series Z-axis piezo stage
- Tokai Hit stage-top incubation system
NIS-Elements high content image acquisition and analysis software