Center of Excellence

Lunenfeld-Tanenbaum Research Institute

Located in the Lunenfeld-Tanenbaum Research Institute of Sinai Health, Canada’s first Nikon Centre of Excellence seeks to provide access to the latest in imaging technologies to its trainees, visiting scholars, and collaborators at the University of Toronto, the greater Toronto community and around the world. Our goal is to harness the power of advanced imaging and image analysis to address the most salient questions in cell biology and to foster meaningful relationships between our researchers and Nikon’s hardware and software development teams.

Contact

Director

Laurence Pelletier, Ph.D.

Address

Lunenfeld-Tanenbaum Research Institute
Sinai Health
600 University Avenue Room 1070A
Toronto, ON Canada M5G 1X5

Website

Pelletier Lab

Systems Available

A1R MP Resonant Scanning Multiphoton System

This system features the A1R MP resonant scanning multiphoton system with visible light confocal capabilities, based on the Ti2-E inverted microscope with stage-top incubation chamber, for a variety of live and fixed specimen imaging applications.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
  • A1R MP resonant scanning multiphoton system
  • Coherent Chameleon Discovery dual beam laser unit (680-1300nm)
  • Episcopic GaAsP non-descanned detectors (NDDs)
  • DU4G GaAsP 4-channel confocal detector
  • Lumencor SOLA light engine
  • Tokai Hit stage-top incubation system

  • NIS-Elements high content image acquisition and analysis software

A1R-HD25 Large Field of View Confocal and CREST X-Light V2 Large Field of View Spinning Disk System

The A1R-HD25 large field of view resonant scanning confocal system and CREST X-LIGHT V2 large field of view spinning disk confocal system are both configured on a Ti2-E inverted microscope, equipped with the stage-top incubation chamber for live cell imaging.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • A1R HD25 high-definition resonant scanning confocal system

  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)

  • DU4G GaAsP 4-channel confocal detector
  • Crest X-Light V2 L-FOV spinning disk confocal system
  • Lumencor Celesta 7-line laser unit (405nm, 446nm, 477nm, 520nm, 546nm, 638nm, 749nm)
  • Photometrics Prime95B 25mm sCMOS camera
  • Lumencor SOLA light engine
  • Tokai Hit stage-top incubation system

  • NIS-Elements high content image acquisition and analysis software

BioPipeline Live High Content Imaging System with A1R-HD25 Resonant Scanning Confocal

The A1R-HD25 large field of view resonant scanning confocal system on a Ti2-E inverted microscope base, equipped with the full enclosure incubation and robotic delivery system for multiwell plates, to enable long-term high content imaging of live samples.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • A1R HD25 high-definition resonant scanning confocal system

  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)

  • DU4G GaAsP 4-channel confocal detector
  • Lumencor Spectra III 8-line light engine
  • 44 multiwell plate capacity incubator
  • Full enclosure incubation system for imaging
  • Robotic system for automated delivery of the plates from the incubator to the microscope stage

  • NIS-Elements high content image acquisition and analysis software

CSU-W1 SoRa Spinning Disk Confocal and Superresolution System

The CSU-W1 SoRa spinning disk with Cairn Research TwinCam optics for simultaneous dual camera imaging, configured on a Ti2-E inverted microscope base, combines confocal and super-resolution capabilities with wide variety of laser lines for fast and gentle multiwavelength imaging.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Yokogawa CSU-W1 SoRa spinning disk confocal
  • LUN-F XL 7-line laser unit (405nm, 440nm, 488nm, 514nm, 561nm, 594nm, 640nm)
  • Cairn Research TwinCam optics for simultaneous dual camera imaging
  • Two Hamamatsu FusionBT sCMOS cameras
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Lumencor SOLA light engine
  • Tokai Hit stage-top incubation system

  • NIS-Elements high content image acquisition and analysis software

CSU-X1 Spinning Disk Confocal System

The CSU-X1 spinning disk confocal system with W-View Gemini emission splitting optics, configured on a Ti2-E inverted microscope base, enables fast and gentle imaging of live and fixed specimens.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Yokogawa CSU-X1 spinning disk confocal

  • Toptica Photonics iChrome MLE 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • Andor Zyla 4.2 sCMOS camera
  • Hamamatsu W-View Gemini image splitting optics
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Lumencor SOLA light engine

  • NIS-Elements high content image acquisition and analysis software

A1R-HD Resonant Scanning Confocal System with High-Speed Widefield Imaging Capabilities

The Nikon A1R-HD resonant scanning confocal system with spectral detection capabilities and equipment for high-speed widefield epifluorescence imaging is integrated on a Ti2-E inverted microscope base, for fast multichannel image acquisition.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • A1R HD high-definition resonant scanning confocal system

  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)

  • DU4G GaAsP 4-channel confocal detector
  • DUV-B 2-channel spectral GaAsP detector
  • Hamamatsu FusionBT sCMOS camera
  • Lumencor Spectra III 8-line light engine
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system

  • NIS-Elements high content image acquisition and analysis software