Software Packages

NIS-Elements Advanced Research

Optimized for advanced research applications.

Nikon’s flagship NIS-Elements AR software integrates fully automated image acquisition, advanced device control, and powerful analysis and visualization tools. It supports 6D imaging and offers a comprehensive suite of capabilities, including volume rendering, movie creation, image processing, and archiving.

Acquire

From Single to Multi-Dimensional imaging
- Intuitive display of multi-dimensional datasets with multiple viewing options (e.g. slice, volume, time, spectral, binary, etc.), easy data management, and export-ready
Go Beyond Image Capture
- Photo-Stimulation
- Perfusion
- Realtime measurements (e.g. Ca²⁺, FRET) during image acquisition
Image without Constraints or Limitations with JOBS
- Custom-build your acquisition workflow with graphical programming
Intelligent Acquisition with JOBS and General Analysis (GA)
- Let the results dictate the acquisition parameters with conditional workflows
- Only acquire meaningful data
- Reduce time spent on data mining
Maximized Device Performance
- NIS-Elements is designed to optimize the speed at which all components can perform
- Maximize device usage through hardware triggering
- Enables fastest possible image capture times for imaging and tracking fast biological events
- Eliminates unnecessary light exposure, extending the health of the sample for long-term time lapses
Largest 3^rdparty integrator in the industry
- Easily integrate and control non-Nikon devices for fully customized experiments
- Quickly adapt to changing research needs by utilizing 3^rdparty devices

Quantitate

From simple manual measurements to automatic data collection of hundreds of parameters
Full suite of binary and image processing tools in General Analysis (GA)
Multi-channel binary software assay toolbox
2D and 3D Tracking and Measurement
Realtime analysis of live cell dynamics during acquisition
- Intensity Based Measurements
- Morphological Based Measurements
- Object Tracking

Process and Visualize

Easily view tiled/stitched large images
Unique Extended Depth of Focus (EDF) view for creating beautiful, high-contrast, in-focus 2D projection images from 3D data (option)
Advanced 3D and 2D deconvolution algorithms for extending resolution
Unique visualization options such as XYT view (3D kymograph)

Publish

Mulitple Image formats
Advanced movie creator with annotations
Easily combine images and analysis results to create beautiful figures
Data export to Excel, Matlab or any third party or customer software

NIS-Elements Basic Research

Geared for acquisition and device control for standard research applications requiring four-dimensional imaging.

Developed for standard research applications such as analysis and photodocumentation of fluorescent imaging, NIS-Elements BR features up to four-dimensional acquisition and advanced device control capabilities.

Acquisition and device control for standard research applications

Basic Multidimensional Imaging
Integration of all moving parts so you can focus on science and data
Measurement
- Intensity, ROIs, Counting, Morphology
Large Image Stitching
Show Multi-Channel Data & Timelapse
Unique Extended Depth of Focus (EDF) view for creating beautiful, high-contrast, in-focus 2D projection images from 3D data (option)

NIS-Elements Documentation

Dedicated photo‑documentation software package with basic measuring and reporting tools.

NIS-Elements D is a documentation viewer allowing users to perform routine “point-and-shoot” image acquisition, manual measurements, and optional automated object counting.

Easy capture, processing, and storage

Easy camera controls and presets
Flexible imaging: Automated XY, Z control, or Timelapse
Manual and Automated Measurement
- Counting and morphology
Annotate your datasets
Unique Extended Depth of Focus (EDF) view for creating beautiful, high-contrast, in-focus 2D projection images from 3D data (option)
High Dynamic Range (HDR) image acquisition combines images acquired with different exposure times to capture the full dynamic range of the specimen in one image (option)
Supports custom workflow using macros
Single document interface. Does not support fluorescence imaging nor wavelength switching

Note: NIS-Elements D is not for clinical diagnostic use.

NIS-Elements Confocal & Enhanced Resolution

Integrates confocal specific acquisition controls and advanced image analysis and visualization functionality.

Dedicated interface for Nikon’s confocal and multiphoton systems, providing easy instrument setup and streamlined operation. Incorporates many of the features of NIS-Elements AR for advanced acquisition, image processing, analysis, visualization and data sharing capability.

Resolution

Image resolution is defined as the smallest distance between 2 points that can be resolved. The theoretical limit of resolution for a conventional optical microscope is approximately 200 nm. Higher resolution images can be theoretically achieved with confocal microscopes, but this has not been effectively achieved. Resolution can be increased beyond the conventional confocal image resolution (up to ~1.5 times improvement in XY; up to ~1.7 times improvement in Z) by using specific image acquisition and processing techniques.

NIS-Elements High Content

Total acquisition-to-analysis solution for high-content imaging applications.

NIS-Elements HC, designed for High Content applications, offers specialized tools for whole well-plate imaging, heat maps, graphing, and cell-based image analysis. It streamlines high-speed, automated acquisition, review, and analysis of multiple well-plate experiments, with wizards that simplify setup and parameter definition, including plate configuration, handling, autofocusing, filter switching, and detector control.

Speed and Flexibility

Streamlines high-speed, automated well-plate acquisition, data review, analysis and management of multiple well plate experiments.

NIS-Elements HC interface simplifies experiment setup using wizards. Easily define acquisition parameters including well-plate configuration, plate handling, autofocusing, filter switching and detectors.

① Define general job parameters
• Z-stack
• Sample labelling
• Autofocus
• Sending task completion by e-mail or SNS

② Define optical configurations for image capture

③ Well plate setting
• Define well plate to use
• Select well plate for image capture
• Define XY image capture pattern inside a well
• Sample labelling

④ Define analysis

Review

Realtime viewing of data acquisition and analysis progress for instant inspection. Multiple analysis assays can be run simultaneously during the imaging phase or run post-acquisition on offline stations.

Analysis

Heat maps of well plates, sample images, binary masks, assay results, sample labels and other metadata are centralized for quick filtering, gating and drill down to cellular detail.

Graphing

Create graphs instantly for data review. Classify, filter, tile, label data points from several different graph types. NIS-Elements offers histogram, scatterplot, bar chart, XY line, classification and gating functions. Easily navigate within the Plate View and export to Excel or bitmap.