Stimulated Emission Depletion Super-Resolution Imaging (STED)
STEDYCON allows imaging to nanoscopic scales with over 6x higher lateral resolution compared to a widefield microscope, using direct imaging without the need for reconstruction or post-processing.
Achieve XY resolution better than 40nm using the same familiar labelling protocols used for epifluorescence and confocal imaging.
Key Features
Compact System with <40nm resolution
Expand the capabilities of imaging with a dedicated STED instrument, or add STED capability to an existing microscope imaging system such as a spinning disk or point scanning confocal with plug-and-play ease, extreme stability, and acquire images with better than 40 nm lateral resolution.
The lasers of the STEDYCON are aligned by design by a patented easySTED optical arrangement, making the instrument always imaging-ready; no calibration routines are required.
2 Color STED Imaging
STEDYCON supports imaging in up to 4 confocal channels and 2 STED channels, in multidimensional acquisition (X,Y,Z and timelapse).
When combined with NIS-Elements: denoising, deconvolution, 2D and 3D analysis, tracking, and more are possible.
STEDYCON
Confocal
Nuclear pores and golgiapparatus.
STEDYCON
Confocal
Hippocampal neurons labeled for alpha-Adducin and beta II spectrin.
DynamicPLUS
DynamicPLUS feature offers unrivaled performance with both STED and confocal applications. The avalanche photo detectors (APDs) have a superior quantum efficiency of up to 69% (@ 650nm). This means that even when signal-levels are low, APDs still collect plenty of photons for a meaningful image.
STED imaging and experiments with low labeling densities designed to stay close to physiological conditions are typical. At the same time, the dead-time compensation of DynamicPLUS allows crisp images of high-signal samples, such as bright confocal images.
STEDYFOCUS Module
The new push-button STEADYFOCUS module is a continuous laser-based hardware focus lock for the STEDYCON, guaranteeing confocal and STED imaging over long time lapse without focus drift.
No additional optics are required in the imaging beam path, making it 100% free of fluorescence losses or imaging distortions.
The STEADYFOCUS module is compatible with a multitude of immersion objective lenses and embedding media, for Ti2-E, Ni, and FN1.
