A Seamless Evolution of Your Microscopy
DeepSIM super-resolution technology is based on multi-spot lattice structured illumination and provides a reliable and affordable solution to study subcellular structures with an XY resolution of 100 nm. The DeepSIM super-resolution system is easy-to-use and enables scientists to access deep data from their biological samples without requiring any special sample preparation or dyes.
The DeepSIM relies on a robust multi-spot lattice SIM technique reaching 100 nm XY resolution; a 2-fold increase in spatial resolution compared to Widefield microscopy.
The DeepSIM is designed to work with samples with thicknesses comparable to those used in confocal microscopy, giving super-resolved data over 50 µm depth in non-clarified samples. In this way, even native, heterogeneous, and complex samples can be investigated more thoroughly.
Multi-spot lattice SIM is ideal for fast live imaging
DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024), allowing biological changes to be tracked at cellular and subcellular levels. Moreover, delicate specimens can be thoroughly explored as the DeepSIM minimizes light exposure and therefore the risk of photo-toxicity.
Compatibility with a wide objective magnification range
A two-fold increase in spatial resolution can be obtained using both high magnification (60X, 100X) and low magnification (20X, 40X) objectives. This feature expands the range of capable applications to include complex 3D models such as tissues, organoids, spheroids, and small organisms.
DeepSIM operates across the entire excitation wavelength spectrum from 400 to 750 nm in order to provide maximum flexibility in fluorophore choice and optimal multichannel imaging without spectral overlap.
CrestOptics acknowledges and respects that the images in these galleries have been published with the permission of their authors.