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A Seamless Evolution of Your Microscopy

DeepSIM super-resolution technology is based on multi-spot lattice structured illumination and provides a reliable and affordable solution to study subcellular structures with an XY resolution of 100 nm. The DeepSIM super-resolution system is easy-to-use and enables scientists to access deep data from their biological samples without requiring any special sample preparation or dyes.

Key Features




Microglia cell (tubulin staining shown in green)
(100x oil 1.45 NA objective)

The DeepSIM relies on a robust multi-spot lattice SIM technique reaching 100 nm XY resolution; a 2-fold increase in spatial resolution compared to Widefield microscopy.

Sample Thickness

The DeepSIM is designed to work with samples with thicknesses comparable to those used in confocal microscopy, giving super-resolved data over 50 µm depth in non-clarified samples. In this way, even native, heterogeneous, and complex samples can be investigated more thoroughly.

Mouse brain tissue section with GFP-expressing neurons. A volume acquisition of 130 um is shown in Depth-code visualization.

Cleared mouse intestine tissue, nuclei and blood vessels are shown in red and green, respectively. A 3D movie of 125 um acquisition is shown.

Multi-spot lattice SIM is ideal for fast live imaging

Lysosome dynamics in HeLa cells. 60x 1.4 NA oil objective

Chromosome segregation during mitotic division in HeLa cells. 60x 1.4 NA oil objective

DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024), allowing biological changes to be tracked at cellular and subcellular levels. Moreover, delicate specimens can be thoroughly explored as the DeepSIM minimizes light exposure and therefore the risk of photo-toxicity.

Compatibility with a wide objective magnification range

A two-fold increase in spatial resolution can be obtained using both high magnification (60X, 100X) and low magnification (20X, 40X) objectives. This feature expands the range of capable applications to include complex 3D models such as tissues, organoids, spheroids, and small organisms.

Chromosome spread image obtained with 100x magnification

Human brain organoid image obtained with 20x magnification

Caenorhabditis elegans image showing sensory neurons and interneurons obtained with 60x magnification

Fluorophore spectrum

DeepSIM operates across the entire excitation wavelength spectrum from 400 to 750 nm in order to provide maximum flexibility in fluorophore choice and optimal multichannel imaging without spectral overlap.

3D volume view of a mouse brain tissue section showing neurons with dendritic spines (green), microglia (red), astrocytes (white) and DNA (cyan)

CrestOptics acknowledges and respects that the images in these galleries have been published with the permission of their authors.