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Nikon Imaging Center

UC San Diego

Mission

  • To enhance basic research by providing access to state-of-the-art microscopy and imaging equipment
  • To provide expert technical consultation and support in every step of the imaging process from sample preparation to data analysis and publication
  • To provide training on light microscopy techniques and sample preparation for the UC San Diego community
  • To bring innovative light microscopy and imaging techniques to UC San Diego researchers
  • To enhance connections between UC San Diego and our local biotech and pharma partners 
  • To serve as a learning and demonstration center for our corporate partners and contributors

Contact

NIC Director

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Address

Nikon Imaging Center
Leichtag 483
9500 Gilman Drive; Mail code 0694
University of California, San Diego
La Jolla, CA 92093-0694

Website

UC San Diego

Systems Available

Large FOV Widefield System

Cyril is a widefield system for large field of view (25mm) acquisition with both monochrome and color CMOS cameras.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Large format DS-Qi2 monochrome CMOS camera

  • Large format DS-Ri2 color CMOS camera

  • Okolab stage-top incubation system

  • NIS-Elements software

  • Lumencor SPECTRA X light engine
  • Nikon Instruments NI-BB Triggered Device Hub

C2 Point Scanning Confocal System

Ray is a point-scanning confocal system for both live and fixed samples, featuring high-sensitivity GaAsP detectors and stage-top incubation.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • C2 point scanning confocal system

  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)

  • DU3 3-channel confocal detector
  • DUV-B 2-channel spectral GaAsP detector
  • Lumencor SOLA light engine
  • Tokai Hit stage-top incubation system with oxygen regulation

  • NIS-Elements software

High Content Analysis (HCA) System

Krieger is a high content screening system featuring X-Light V2 large field of view spinning disk confocal scanner and robotic plate loader.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Prior plate-loading robot
  • Crest X-Light V2 L-FOV spinning disk confocal system
  • Lumencor CELESTA light engine
  • Photometrics Iris 15 CMOS camera
  • TI2-N-WID Water Immersion Dispenser

  • NIS-Elements software

N-STORM/TIRF/CSU-X1 Super-Resolution & Spinning Disk Confocal System

Sterling is a multimodal system featuring super-resolution STORM, TIRF, spinning disk confocal, and a DMD module for stimulation experiments.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Yokogawa CSU-X1 spinning disk confocal

  • N-STORM super-resolution single molecule localization microscopy system

  • Lumencor SOLA light engine
  • Agilent MLC400B 4-line laser unit (405nm, 488nm, 561nm, 647nm)
  • Photometrics Prime95B sCMOS camera
  • Andor iXon 897 EMCCD camera
  • Mightex Polygon400 DMD for patterned illumination (Lumencor AURA II light engine)
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system

  • NIS-Elements software

N-SIM/A1R HD Super-Resolution & Confocal System

Lana features an A1R HD resonant scanning confocal and N-SIM super-resolution structured illumination systems for finding the perfect balance between speed and resolution.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • N-SIM super-resolution structured illumination microscopy system

  • A1R HD high-definition resonant scanning confocal system

  • A1-DUS 32-channel spectral detector
  • DU4G GaAsP 4-channel confocal detector
  • Lumencor SOLA light engine
  • Andor iXon 897 EMCCD camera
  • LUN-V 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Okolab stage-top incubation system

  • NIS-Elements software