Center of Excellence
University of Arizona Cancer Center
Located at one of the nation’s leading NCI-designated comprehensive cancer facilities, the Nikon Center of Excellence at the University of Arizona Cancer Center is a Microscopy Shared Resource (MSR) designed to deliver advanced imaging capabilities to accelerate basic and translational cancer research. The Center empowers scientists to visualize and quantify complex cellular and molecular dynamics with exceptional clarity assisting breakthroughs in tumor biology, immuno-oncology, therapy resistance, and precision medicine.
Contact
CofE Director
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Address
University of Arizona Cancer Center3838 N Campbell Ave.
Tucson, AZ 85719
Systems Available
AX R MP Multiphoton
The AX R MP is Nikon’s newest large format multiphoton microscope ideal for deep imaging. The highly improved 2K resonant scanner allows users to acquire high resolution images of fast in vivo events. It is combined with visible wavelengths and tunable emission detectors for maximum capabilities.
Components
- ECLIPSE Ti2-E inverted microscope with Perfect Focus System
- AX R MP hybrid scanner with 2K resonant
- Laser 1: InSight X3+ Tunable Ti:S Laser: 820nm - 1300nm
- Laser 2: InSight X3+ Fixed Laser: 1045nm
- GaAsP non-descanned (NDD) detectors
- Piezo stage with 800um travel
- Capable of SHG (Second-Harmonic Generation) imaging
- NIS-Elements Software
AX R Confocal
The AX R is Nikon’s latest generation laser scanning confocal system with a 25mm field of view, capable of 2K resonant scanning capabilities. The highly sensitive hybrid detectors allows for fast and gentle imaging of sensitive samples while significantly improving the time of data acquisition of fixed specimens.
Components
- ECLIPSE Ti2-E inverted microscope with Perfect Focus System
- AX R hybrid scanner with 2K resonant
- 8-laser line unit (405/445/488/515/561/594/640/730nm)
- DUX-VB4 4ch confocal fixed/tunable detector
- Transmitted detector
- Piezo stage with 600um travel for precise, high-speed z-stack imaging
- WID automatic water dispenser
- NIS-Elements Software
Yokogawa CSU-W1 SoRa Spinning Disk System
Utilizing optical reassignment on a spinning disk system, the CSU-W1 SoRa achieves sub-diffraction limited optical resolution while preserving sample health. It is equipped with a 7 line laser, enabling flexible imaging across a wide variety of fluorophores.
Components
- ECLIPSE Ti2-E inverted microscope with Perfect Focus System
- Yokogawa CSU-W1 SoRA spinning disk
- Photometrics Kinetix sCMOS camera
- 4-laser line unit (405/488/561/640nm)
- 600nm Piezo Z
- Opti-Microscan Galvano Scanner for Photomanipulation
- NIS-Elements Software
Crest Spinning Disk Confocal Microscope
Utilizing a CrestOptics X-Light V2 spinning disk integrated with a Nikon ECLIPSE Ti2 and a Photometrics Prime 95B camera, this system is ideal for any experiment where imaging speed and sample health is a priority.
Components
- Fully motorized ECLIPSE Ti2-E with Perfect Focus System and 4-channel epifluorescence
- Crest spinning disk with Lumencor Celesta 7-line solid state laser light source
- Photometrics Prime 95B sCMOS camera
- 4X-60X objectives with DIC and Phase
- NIS-Elements with high content acquisition tools
N-STORM Super-Resolution System
The Nikon N-STORM system provides several modes of high-resolution modalities for imaging dynamic cellular events with a high signal/noise ratio and single-molecule interactions as low as 20nm lateral resolution.
Components
- ECLIPSE Ti2-E inverted microscope with Perfect Focus System
- Motorized TIRF module
- High-sensitivity Andor iXon EMCCD camera
- 4-laser line unit (405/488/561/640nm)
- NIS-Elements Software
BioPipeline Slide High-Content Microscope
The BioPipeline SLIDE system utilizing the Marzhauser Slide Express 2 loader provides an extremely robust and stable platform for automated slide scanning.