University of Arizona Cancer Center

Opening: Wednesday, April 23, 2025; From left to right: Marco Padilla-Rodriguez, Microscopy Shared Resource (MSR) Manager; Mario Romero-Ortega, Department Head of Biomedical Engineering; Sara Parker, Nikon Instruments, Advanced Imaging Specialist; Swarna Ganesh, Assistant Professor of Biomedical Engineering, hired from the UACC Cancer Engineering partnership; Andy Davis, Nikon Instruments, Senior Director, Sales & Marketing; Ghassan Mouneimne, Microscopy Shared Resource and Nikon CoE Director, Professor of Cellular and Molecular Medicine; Dan Theodorescu, M.D., Ph.D., Director of the UA Cancer Center; Jennifer Barton, Director of BIO5 Institute, Professor of Biomedical Engineering; Brian Craze, Nikon Instruments, Senior Biosystems Applications Manager; Vincent Haile, Nikon Instruments, Associate General Manager; Patrick Keenan, Nikon Instruments, Regional Sales Manager; Andrew Brumm, Nikon Instruments, Advanced Biosystems Manager

Contact

CofE Director

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Address

University of Arizona Cancer Center
3838 N Campbell Ave.
Tucson, AZ 85719

Systems Available

AX R MP Multiphoton

The AX R MP is Nikon’s newest large format multiphoton microscope ideal for deep imaging. The highly improved 2K resonant scanner allows users to acquire high resolution images of fast in vivo events. It is combined with visible wavelengths and tunable emission detectors for maximum capabilities.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
AX R MP hybrid scanner with 2K resonant
Laser 1: InSight X3+ Tunable Ti:S Laser: 820nm - 1300nm
Laser 2: InSight X3+ Fixed Laser: 1045nm
GaAsP non-descanned (NDD) detectors
Piezo stage with 800um travel
Capable of SHG (Second-Harmonic Generation) imaging
NIS-Elements Software

AX R Confocal

The AX R is Nikon’s latest generation laser scanning confocal system with a 25mm field of view, capable of 2K resonant scanning capabilities. The highly sensitive hybrid detectors allows for fast and gentle imaging of sensitive samples while significantly improving the time of data acquisition of fixed specimens.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
AX R hybrid scanner with 2K resonant
8-laser line unit (405/445/488/515/561/594/640/730nm)
DUX-VB4 4ch confocal fixed/tunable detector
Transmitted detector
Piezo stage with 600um travel for precise, high-speed z-stack imaging
WID automatic water dispenser
NIS-Elements Software

Yokogawa CSU-W1 SoRa Spinning Disk System

Utilizing optical reassignment on a spinning disk system, the CSU-W1 SoRa achieves sub-diffraction limited optical resolution while preserving sample health. It is equipped with a 7 line laser, enabling flexible imaging across a wide variety of fluorophores.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
Yokogawa CSU-W1 SoRA spinning disk
Photometrics Kinetix sCMOS camera
4-laser line unit (405/488/561/640nm)
600nm Piezo Z
Opti-Microscan Galvano Scanner for Photomanipulation
NIS-Elements Software

Crest Spinning Disk Confocal Microscope

Utilizing a CrestOptics X-Light V2 spinning disk integrated with a Nikon ECLIPSE Ti2 and a Photometrics Prime 95B camera, this system is ideal for any experiment where imaging speed and sample health is a priority.

Components

Fully motorized ECLIPSE Ti2-E with Perfect Focus System and 4-channel epifluorescence
Crest spinning disk with Lumencor Celesta 7-line solid state laser light source
Photometrics Prime 95B sCMOS camera
4X-60X objectives with DIC and Phase
NIS-Elements with high content acquisition tools

N-STORM Super-Resolution System

The Nikon N-STORM system provides several modes of high-resolution modalities for imaging dynamic cellular events with a high signal/noise ratio and single-molecule interactions as low as 20nm lateral resolution.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
Motorized TIRF module
High-sensitivity Andor iXon EMCCD camera
4-laser line unit (405/488/561/640nm)
NIS-Elements Software

BioPipeline Slide High-Content Microscope

The BioPipeline SLIDE system utilizing the Marzhauser Slide Express 2 loader provides an extremely robust and stable platform for automated slide scanning.

Components

High-throughput imaging service
High-NA objective lenses from 2X - 40X
7 laser line unit (390/440/475/510/555/637/748)
Hamamatsu ORCA-Flash4.0 V3 camera

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