Nikon Imaging Center
UC San Diego
Mission
- To enhance basic research by providing access to state-of-the-art microscopy and imaging equipment
- To provide expert technical consultation and support in every step of the imaging process from sample preparation to data analysis and publication
- To provide training on light microscopy techniques and sample preparation for the UC San Diego community
- To bring innovative light microscopy and imaging techniques to UC San Diego researchers
- To enhance connections between UC San Diego and our local biotech and pharma partners
- To serve as a learning and demonstration center for our corporate partners and contributors
Contact
NIC Director
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Address
Nikon Imaging CenterLeichtag 483
9500 Gilman Drive; Mail code 0694
University of California, San Diego
La Jolla, CA 92093-0694
Website
Systems Available
Large FOV Widefield System
Cyril is a widefield system for large field of view (25mm) acquisition with both monochrome and color CMOS cameras.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Large format DS-Qi2 monochrome CMOS camera
Large format DS-Ri2 color CMOS camera
- Okolab stage-top incubation system
NIS-Elements software
- Lumencor SPECTRA X light engine
- Nikon Instruments NI-BB Triggered Device Hub
C2 Point Scanning Confocal System
Ray is a point-scanning confocal system for both live and fixed samples, featuring high-sensitivity GaAsP detectors and stage-top incubation.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
C2 point scanning confocal system
LUN-4 4-line laser unit (405 nm, 488 nm, 561 nm, 640 nm)
- DU3 3-channel confocal detector
- DUV-B 2-channel spectral GaAsP detector
- Lumencor SOLA light engine
- Tokai Hit stage-top incubation system with oxygen regulation
NIS-Elements software
High Content Analysis (HCA) System
Krieger is a high content screening system featuring X-Light V2 large field of view spinning disk confocal scanner and robotic plate loader.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Prior plate-loading robot
- Crest X-Light V2 L-FOV spinning disk confocal system
- Lumencor CELESTA light engine
- Photometrics Iris 15 CMOS camera
- TI2-N-WID Water Immersion Dispenser
NIS-Elements software
N-STORM/TIRF/CSU-X1 Super-Resolution & Spinning Disk Confocal System
Sterling is a multimodal system featuring super-resolution STORM, TIRF, spinning disk confocal, and a DMD module for stimulation experiments.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Yokogawa CSU-X1 spinning disk confocal
N-STORM super-resolution single molecule localization microscopy system
- Lumencor SOLA light engine
- Agilent MLC400B 4-line laser unit (405nm, 488nm, 561nm, 647nm)
- Photometrics Prime95B sCMOS camera
- Andor iXon 897 EMCCD camera
- Mightex Polygon400 DMD for patterned illumination (Lumencor AURA II light engine)
- Mad City Labs Nano-Z series Z-axis piezo stage
- Tokai Hit stage-top incubation system
NIS-Elements software
N-SIM/A1R HD Super-Resolution & Confocal System
Lana features an A1R HD resonant scanning confocal and N-SIM super-resolution structured illumination systems for finding the perfect balance between speed and resolution.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- N-SIM super-resolution structured illumination microscopy system
A1R HD high-definition resonant scanning confocal system
- A1-DUS 32-channel spectral detector
- DU4G GaAsP 4-channel confocal detector
- Lumencor SOLA light engine
- Andor iXon 897 EMCCD camera
- LUN-V 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Mad City Labs Nano-Z series Z-axis piezo stage
- Okolab stage-top incubation system
NIS-Elements software