![](https://www.microscope.healthcare.nikon.com/images/mastheads/Imaging-Centers/northwestern_masthead.jpg)
Nikon Imaging Center
Northwestern University
The Nikon Imaging Center at Northwestern University Feinberg School of Medicine (NIC@NU-FSM) is an integral component of the Northwestern University Cell Imaging Facility, supported by Nikon, the Feinberg School of Medicine, Department of Cell & Molecular Biology and the Robert H. Lurie Comprehensive Cancer Center.
Established in 2008, the NIC@NU-FSM carries an important mission to:
- Augment basic research by providing access to state-of-the-art imaging equipment
- Provide training courses and organize symposia on light microscopy techniques for the benefits of the Northwestern community as well as the greater Chicago area
- Introduce cutting-edge imaging technology to Northwestern University
- Serve as a instrument evaluation and testing site for new equipment from Nikon
- Serve as a learning center for staff, researchers and students
![](https://www.microscope.healthcare.nikon.com/images/product-logos/Nortwestern-Logo.png)
Contact
NIC Director
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Address
Nikon Imaging CenterMorton 2-633
Feinberg School of Medicine
303 E. Chicago Avenue
Chicago, IL 60611
Website
Systems Available
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7200/Northwestern-N-SIM1_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
N-SIM Super-Resolution System
Featuring both Nikon's N-SIM structured illumination system and incubation, this system provides a powerful platform for live-cell super-resolution imaging.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
- N-SIM super-resolution structured illumination microscopy system
- LU5 N-SIM 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- Tokai Hit stage-top incubation system
NIS-Elements software
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7207/Northwestern-Biostation2_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
BioStation IM-Q System
The BioStation IM-Q incorporates a microscope, an incubator and a high-sensitivity cooled CCD camera in a compact body. This all-in-one package provides a stable environment for live cells and advanced solutions for simple long-term time-lapse data acquisition.
Components
- BioStation IM-Q System
- Built-in incubation
- Phase Contrast and Fluorescence observation methods
- Built-in cooled monochrome camera
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7207/Northwestern-Biostation2_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
BioStation IM-Q System
The BioStation IM-Q incorporates a microscope, an incubator and a high-sensitivity cooled CCD camera in a compact body. This all-in-one package provides a stable environment for live cells and advanced solutions for simple long-term time-lapse data acquisition.
Components
- BioStation IM-Q System
- Built-in incubation
- Phase Contrast and Fluorescence observation methods
- Built-in cooled monochrome camera
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7216/Northwestern-Biostation-CT_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
BioStation CT System
The BioStation CT combines the cell culture and imaging environments to enable accurate monitoring of individual cells and colonies while maintaining stable environmental conditions.
Components
- BioStation CT System
- Built-in incubation
- Robotic plate loader, up to 30 culture vessels
- Built-in LCD touchscreen controller
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7222/Northwestern-AZ-C2_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
AZ-C2 Macro Confocal System
The AZ/C2 is a macro confocal microscope system which allows for the capture of confocal images allowing for better resolution of 3D specimens.
Components
- AZ100 multizoom microscope
C2 point scanning confocal system
LUN-4 4-line laser unit (408nm, 488nm, 561nm, 640nm)
- DU3 3-channel confocal detector
- Qimaging Retiga 2000 scientific CCD camera
- Excelitas X-Cite XLED1 LED illumination system
NIS-Elements software
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7229/Northwestern-A1RMP_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
A1R MP Resonant Scanning Multiphoton System
Deep tissue imaging is made possible using this Nikon A1R MP multiphoton system configured on Ni-E upright microscope.
Components
ECLIPSE Ni-E upright microscope
- Nikon A1R MP resonant scanning multiphoton system
- Coherent Ti:S Chameleon Vision-S laser (tunable 690-1050nm)
- Episcopic GaAsP and diascopic non-descanned detectors (NDDs)
- Tokai Hit stage-top incubation system
- Mad City Labs Nano-Z series Z-axis piezo stage
NIS-Elements software
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7232/Northwestern-N-STORM-TIRF-CSU_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
N-STORM/TIRF/CSU-X1 Super-Resolution & Spinning Disk Confocal System
This multimodal TIRF, super-resolution N-STORM, and spinning disk confocal system is adaptable to a variety of samples.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
N-STORM super-resolution single molecule localization microscopy system
Yokogawa CSU-X1 spinning disk confocal
- LUN-V 6-line laser unit (405nm, 458nm, 488nm, 515nm, 561nm, 640nm)
- Andor iXon 897 EMCCD camera
- Tokai Hit stage-top incubation system
NIS-Elements software
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/8249/Northwestern_CSU_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
TIRF/FRAP/CSU-W1 Spinning Disk Confocal System
Combining the Nikon Ti2-E microscope with Yokogawa CSU-W1 spinning disk and emission splitting optics unlocks fast and large field of view confocal imaging.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Yokogawa CSU-W1 spinning disk confocal
- Nikon TIRF illuminator
- Bruker Mini-Scanner galvo-based stimulation system
- LUN-V 6-line laser unit (405nm, 458nm, 488nm, 515nm, 568nm, 640nm)
- MAG Biosystems Dual-Cam two-camera emission splitter
- 2x Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera (used with emission splitter)
- Photometrics Prime95B sCMOS camera
- Tokai Hit stage-top incubation system
NIS-Elements software
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7236/Northwestern-Ti2-E_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
Inverted Widefield Microscope System
This fast and large field of view widefield system features a Nikon Ti2-E and Lumencor Spectra III light engine.
Components
ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Large format DS-Qi2 monochrome CMOS camera
- Lumencor SPECTRA III light engine
- Tokai Hit stage-top incubation system
NIS-Elements software
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/NIC-Systems/7239/Northwestern-A1Rsi_d5bf01b6690ca8c589d10eb51a9ecfd9.jpg)
A1Rsi Resonant Scanning Confocal System
Using the Nikon A1Rsi resonant scanning confocal system with spectral detector, this system is made for linear unmixing of multiplynlabeled samples.
Components
- ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
- A1Rsi resonant scanning confocal system
- LU4A 6-line laser unit (405nm, 458nm, 488nm, 515nm, 561nm, 638nm)
- A1-DUS 32-channel spectral detector
- DU4G GaAsP 4-channel confocal detector
NIS-Elements software