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NIS-Elements

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Nikon’s Confocal NIS-Elements Package

Dedicated interface for Nikon’s confocal and multiphoton systems, providing easy instrument setup and streamlined operation.  Incorporates many of the features of NIS-Elements AR for advanced acquisition, image processing, analysis, visualization and data sharing capability.

Key Features

Enhance your Confocal Resolution with ER

Higher resolution confocal images can be easily generated with a single click. The software assesses the captured image and automatically determines processing parameters to achieve enhanced resolution.

ER can be applied to previously captured confocal images as well.

Resolution

Image resolution is defined as the smallest distance between 2 points that can be resolved. The theoretical limit of resolution for a conventional optical microscope is approx. 200nm. Higher resolution images can be theoretically achieved with confocal microscopes, but this has not been effectively achieved. Using unique image processing technology, image resolution can be increased beyond that of a conventional confocal image (~ 1.5 times improvement in XY; ~1.7 times improvement in Z).

Standard confocal image

Enhanced Resolution image

Red: Central spindle, Blue: Nuclei
Image courtesy of: Toshinori Hyodo Ph.D., Department of Biochemistry, Aichi Medical University School of Medicine

Standard confocal image

Enhanced Resolution image

Stress Fibers (LLC-PK1, Pig Kidney Cells), Green: F-actin, Red: Myosin Heavy Chain
Image courtesy of: Keiju Kamijo Ph.D., Division of Anatomy and Cell Biology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University

Standard confocal image

Enhanced Resolution image

Apical surfaces of auditory epithelia of mouse cochleae were stained by Atto-565-phalloidin at postnatal day 2.
Image courtesy of: Dr. Hideru Togashi, Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine.

Zebrafish lens at 5 dpf. Nuclei (green) and actin filaments (red) are visualized with Cytox-green and Rhadamine-conjugated phalloidin, respectively. High magnification images indicate lens fiber cells, which become flat and stack against each other.

Image courtesy of: Drs. Toshiaki Mochizuki and Ichiro Masai, Developmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University

Upgradability

Enhanced Resolution mode is compatible with A1 HD25/A1R HD25 confocal microscopes. An upgrade plan is available for current NIS-Elements C users.