Image courtesy of Laurence Pelletier Lab, LTRI

A1 HD25 / A1R HD25

Confocal Microscope System

Discontinued Replaced by AX / AX R with NSPARC


A1 HD25 A1R HD25
Scan head input/output port 1 laser input port
2 signal output ports for standard, spectral and optional detector*1
FOV Ti2-E:Square inscribed in a Ø25 mm circle
Ni-E/FN1:Square inscribed in a Ø18 mm circle
Image bit depth 4096 gray intensity levels (12 bit)
Recommended installation conditions Temperature 23 ± 5 ℃, humidity 70 % (RH) or less (non-condensing)
Z step Ti2-E: 0.01 μm, 0.02 μm (with encoder control), FN1 stepping motor: 0.05 μm, Ni-E: 0.025 μm
Compatible microscopes ECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope,
ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type)
Laser A1 HD25 A1R HD25
LU-N3 3-laser unit 405 nm, 488 nm, 561nm lasers are installed; built-in AOTF
*Cannot be used with A1-DUS spectral detector
LU-N4/N4S 4-laser unit 405 nm, 488 nm, 561 nm,640 nm lasers are installed; built-in AOTF
*LU-N4 cannot be used with A1-DUS spectral detector
LU-NV series laser unit Compatible lasers : 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm ; built-in AOTF
Standard fluorescence detector A1 HD25 A1R HD25
Wavelength 400-750 nm
Detector A1-DU4-2 4 Detector Unit: 4 Multi-Alkali PMTs
A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
Filter cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75
Diascopic detector (Option) A1 HD25 A1R HD25
Wavelength 485-650 nm
Detector Multi-Alkali PMT
Scan head A1 HD25 A1R HD25
Standard image acquisition Scanner: galvano scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed:
Standard mode: 1.4 fps (512 x 512 pixels, bi-direction, 0.72x zoom), 2 fps (512 x 512 pixels, bi-direction, 1x zoom)
Fast mode: 10 fps (512 x 512 pixels, bi-direction, 8x zoom), 200 fps (512 x 16 pixels, bi-direction, 8x zoom)*2
Zoom: 0.72-1000x continuously variable
Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z
High-speed image acquisition Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis)
Pixel size: max. 1024 x 1024 pixels
Scanning speed: 15 fps (1024 x 1024 pixels), 30 fps (512 x 512 pixels), 60 fps (256 x 256 pixels) to 720 fps (512 x 16 pixels), 7,800 lines/sec (line speed)
Zoom:0.72x, 0.82x, 0.9x, 1x, 1.2x, 1.5x, 1.75x, 2x, 2.4x, 3x, 4x, 5x, 6x, 7x, 8x
Scan mode: X-Y, X-T, X-Z
Acquisition method: High-speed image acquisition
Dichroic mirror Low-angle incidence method, Number of positions: 8
Standard filter: 405/488/561/640, BS20/80
Optional filter:405/488, 405/488/561, 405/488/543/640, 457/514
Pinhole 12-256 μm variable (1st image plane)
Spectral detector (option) A1 HD25 A1R HD25
A1-DUS spectral detector unit Number of channels: 32
Wavelength detection range: 400 - 750 nm
Spectral image acquisition speed: 4 fps (256 x 256 pixels)
Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode)
Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps
Compatible with galvano scanner only
A1-DUVB-2 GaAsP detector unit Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter
Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest:320 nm
Maximum pixel size: 4096 x 4096 (CB mode/VB mode)
Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps
Compatible with galvano and resonant scanners
Option A1 HD25 A1R HD25
Motorized XYZ Motorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E)
Photostimulation module*3
(for Ti2-E)
XY galvano scanning unit (Light source: LU-N3/N4 Laser Unit)
DMD module (Light source: C-LEDFI Epi-FL LED illuminator, LU-N3/N4 laser unit)
Stimulation form: ROI/line/point
Stimulation mode: Sequential, Simultaneous
Software A1 HD25 A1R HD25
Acquisition/analysis Basic software: NIS-Elements C
Optional software for high-resolution acquisition: NIS-Elements C-ER
Display/image generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Application FRAP, FLIP, FRET(option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computer A1 HD25 A1R HD25
OS Windows 10 Pro 64bit, English or Japanese version, OS Version 1709
Windows 7 Professional, 64bit, SP1 English or Japanese version, Windows Update KB3118401 or later
CPU Intel Xeon W-2125 (4.0GHz, quad core, 8.25 MB, 2666 MHz) or higher
RAM 32GB or 64GB
HDD 1st HP Z Turbo G2 512GB PCIe M.2 SSD
Optical Drive Super Multi drive, up to x16 speed or higher
Graphics NVIDIA Quadro P600 or higher (NIS-Elements C-ER: NVDIA Quadro P4000)
(PCI Express/two-screen split display supported)
Extension slot Two PCI Express 3.0 (x16) slots (one slot to be used for graphics)
One PCI Express 3.0 (x8) slot
Two PCI Express 2.0 (x4) slot
LAN port 10/100/1000 Network/Interface x 2 (for connection to controller, for connection to external LAN)
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended

*1 FCS/FCCS/FLIM is possible in combination with third-party systems
*2 Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.
*3 Adapter for Ti2-LAPP system, light source, hybrid dichroic mirror for photoactivation and imaging, and control board are all required.

Dimensions and weight

Scan Head 277(W) x 163(H) x 366(D) mm Approx. 14.5 kg
Controller 360(W) x 575(H) x 599(D) mm Approx. 40 kg
A1-DU4-2 4 Detector Unit 360(W) x 199(H) x 593.5(D) mm Approx. 16 kg
A1-DUG-2 GaAsP Multi Detector Unit 360(W) x 199(H) x 593.5(D) mm Approx. 16 kg
A1-DUS Spectral Detector Unit 360(W) x 323(H) x 593.5(D) mm Approx. 26 kg
A1-DUVB-2 GaAsP Detector Unit 360(W) x 114(H) x 595.5(D) mm Approx. 10 kg
LU-N4/N4S/N3 Laser Unit 360(W) x 210(H) x 593.5(D) mm Approx. 19 kg
LU-NV Laser Unit 400(W) x 781(H) x 685(D) mm Approx. 70 kg
LU Controller Box B (for LU-NV) 400(W) x 123(H) x 687(D) mm Approx. 7 kg

Power source

Scan Head and Controller Input 100-240V ± 10%, 50-60Hz, 5A-2A
Computer Unit Input 100-240V ± 10%, 50-60Hz, 12A-10A
LU-N4/LU-N4S/LU-N3 Input 100-240V ± 10%, 50-60Hz, 2A max.
LU-NV Series Input 100-240V ± 10%, 50-60Hz, 4.8A max.
LU Controller Box B (for LU-NV) Input 100-240V ± 10%, 50-60Hz, 1A max.
Inverted Microscope Ti2-E and HG Fiber Illuminator Intensilight Input 100-240V ± 10%, 50-60Hz, 6.3A max.

Note: When an air compressor is used with a vibration isolated table, an additional power source is necessary.