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Objectives for imaging cleared specimens

Objectives designed for imaging whole, cleared tissues provide ultra-deep, large-scale images with maximum clarity and contrast.

The CFI90 20XC Glyc is suitable for deep imaging of tissues and whole organs treated with a variety of tissue clearing methods.*1 The objective features a correction collar that is compatible with a wide range of immersion media*2 and refractive indices ranging from 1.44 to 1.50. With its high NA, large field-of-view, and ultra-long working distance, this objective enables observation of large samples and whole organs with exceptional clarity and throughput. The objective provides superior chromatic aberration correction and transmittance in the 700 nm to 1300 nm wavelength range to provide exceptional images for multiphoton applications.

Similarly, the CFI Plan Apochromat 10XC Glyc supports a wide range of refractive indices, enabling observations in water, oil and glycerin as well as various tissue clearing agents.*1 This long working distance, wide field-of-view objective also provides clear, high-contrast images deep inside tissue due to its high numerical aperture, broad chromatic aberration correction and high transmittance Nano Crystal Coat technology. This objective can accommodate cover glass preparations and is also compatible with inverted microscopes.

*1: Some tissue clearing reagents may damage these products. Please confirm with a distributor before purchasing the product.
*2: Not all immersion liquids are supported. Please check with your distributor.

Download Objectives Brochure (8.78MB)


Cover glass thicknessCorrection ringObservation
CFI90 20XC GlycDiagramGraph1.008.20BF*, FL (NIR)
CFI Plan Apochromat 10XC GlycDiagramGraph0.50Upright: 5.50
Inverted: 2.00
0-0.17BF, DF, FL (visible light, NIR)

Note: CFI90 20XC Glyc is compatible with Ni-E/FN1 microscopes with a dedicated nosepiece. It corrects chromatic aberrations from 587 nm upward, enabling this lens to be used as a viewfinder during brightfield observations.

BF: Brightfield
DF: Darkfield
PH: Phase contrast
POL: Simple polarizing
FL: Fluorescence

*Possible but not recommended

**External phase contrast observation is possible with ECLIPSE Ti2-E