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Upright Microscope

Fully motorized and advanced research upright microscope offering automated acquisition capabilities of multi-dimensional images

Eclipse Ni-E, Nikon's top-of-the-line motorized upright microscope, was developed to meet the increasing demands for sophistication and automation of research. Eclipse Ni-E boasts flexible system expandability and supports a broad range of advanced research applications.

With Nikon's proprietary stratum structure, exchangeable focusing mechanisms, from focusing stage to focusing nosepiece, a diverse array of motorized accessories, and automatically changing observation conditions, Ni-E has expanded application possibilities to provide the perfect solution for all advanced bioscience and medical research.

Download Eclipse Ni/Ci Brochure (11.94MB)


Key Features

Stratum structure improves system expandability

Nikon's proprietary stratum structure enables simultaneous mounting of two optical paths on one microscope to support various applications.

This structure allows double layer mounting of an epi-fluorescence illuminator and a barrier filter wheel, or a photoactivation unit and a back port unit, developed for the first time for upright microscopes.

Ni-E (focusing stage) configured with motorized epi-fluorescence illuminator, motorized condenser and motorized quadrocular tilting tube

Ni-E (focusing nosepiece) configured with motorized excitation and barrier filter wheels, motorized epi-fluorescence illuminator, confocal microscope system, high-sensitivity camera, motorized stage and motorized quadrocular tilting tube


High-speed motorized accessories

The wide array of motorized accessories including motorized excitation and emission filter wheels, can be custom-combined depending on application.


Focusing mechanisms can be modified for the application

The microscope can be re-configured by switching the focusing stage and focusing nosepiece, enabling a fixed-stage configuration with motorized focusing to meet the demands of experiments such as in vivodeep imaging utilizing multiphoton confocal microscopy.

Ni-E (focusing nosepiece) configured with multiphoton confocal microscope system, episcopic and diascopic NDD units, motorized epi-fluorescence illuminator and motorized quadrocular tilting tube


Automatic adjustment with objective changeover

Condenser, aperture, field diaphragm and ND filter are automatically set to optimal positions according to the objective magnification in use. In addition, XYZ stage travel distance per handle rotation and parfocal distance deviation correction are automatically adjusted. Microscope settings can also be manually adjusted.


Reproducing observation conditions

Selected observation conditions can be designated to individual buttons, enabling changes to be made at the push of a button. This is particularly convenient when reproducing specific observation conditions.


High-precision motorized focusing

High-precision Z-focus provides accurate Z-position information required for use with confocal laser microscopes and supports automated Z-series acquisition. Individual coarse and fine focus knobs enhance ease of operation.


Supreme optical performance

Nano Crystal Coat technology

Employed for the first time in microscope objectives, this anti-reflective coating consists of nanometer-size particles. It is based on semiconductor manufacturing technology and is also used for Nikon camera lenses. The coating's coarse structure and uniform arrangement of particles in a spongy construction result in extremely low reflective indices.

without nano crystal coat

with nano crystal coat


CFI Plan Apochromat Lambda series objective lens

With remarkably high NA, greatly improved transmission in the long wavelength range thanks to Nikon’s proprietary Nano Crystal Coat technology, and chromatic aberration correction from 435 nm to 850 nm, these objectives are ideally suited not only for brightfield and DIC observations but also for fluorescent observations. These lenses allow bright and clear image acquisition for a broad range of wavelengths from visible light fluorescence to near-IR imaging. Their high-efficiency transmission means minimal excitation light can be used to reduce photo-damage to specimens.


Water dipping objective lenses

With a long working distance and high NA, these objectives provide excellent transmission in near IR wavelength range. The axial chromatic aberration of CFI Apochromat NIR 40X/60X W has been corrected up to 850 nm, providing high-resolution images of minute structures in thick specimens during IR-DIC observation. The CFI75 Apochromat 25XC W series and CFI Plan 100XC W also feature high NA and long working distances. With chromatic aberration corrected in the IR region, these objectives are ideal for multiphoton excitation observation. In addition, these lenses provide correction collars to compensate for temperature- and depth-induced spherical aberration, resulting in crystal clear images of areas deep within thick specimens.


Uniformly bright illumination

A built-in “fly-eye” lens ensures uniform and bright illumination of the field of view, from edge-to-edge, at any magnification.


Fluorescence noise elimination

Nikon's proprietary noise terminator mechanism is employed in the epi-fluorescence cube turret and filter cubes. The S/N ratio is dramatically improved by eliminating stray light in the filter cubes, allowing images with weak fluorescent signals to be captured with high contrast and brightness.


Ultimate ease of operation

Simple digital imaging

Images can be captured with Digital Sight cameras by simply pressing the image capture button located on the microscope base.

Image capture button


3D ergonomic design

Most microscope controls can be operated with easy-to-reach buttons on the front of the Ni-E. Operation buttons on the microscope's sides are angled to allow intuitive touch-type operations during observation.


Controllers support intuitive operations

The DS-L4 camera control unit is a touch-screen, tablet-style interface that can be used to control Ni-E motorized functions and peripheral devices as well as DS cameras, eliminating the additional space requirements of a desktop PC. An ergo-controller with similar operational feel to the actual microscope is also available.

DS-L4 camera control unit

Ergo controller