A1 MP+ / A1R MP+ Multiphoton
High Definition Multiphoton
Discontinued Replaced by AX R MP with NSPARC
Objectives
Highest performance optics for multiphoton imaging
A selection of high numerical aperture (NA) objectives are available, which provide chromatic aberration correction up to the near-infrared range, and enable high-efficiency excitation for multiphoton imaging.
CFI90 20XC Glyc
This objective includes a correction collar that accommodates refractive indices ranging from 1.44 to 1.50 and is compatible with a variety of immersion media and tissue-clearing agents.*1 With its high NA, large field-of-view, and ultra-long working distance, this objective enables observation of large samples and whole organs with exceptional clarity and throughput. It also provides superior chromatic aberration correction and transmittance in the NIR wavelength range for multiphoton applications.
- Working distance: 8.2 mm
- Numerical aperture: 1.00
- Chromatic aberration correction: from 588nm to 1300nm
- Nano Crystal Coat applied.
CFI Plan Apochromat 10XC Glyc
Correction for refractive indices from 1.33 to 1.51 enables deep 3D-imaging of tissues cleared with a variety of optical clearing agents.*1
- Working distance: 5.50 mm
- Chromatic aberration correction: from UV through to near IR
- Nano Crystal Coat applied.
CFI75 Apochromat 25XC W 1300
This lens is perfect for deep multiphoton imaging, achieving both a high NA and long working distance, as well as correcting spherical aberrations caused by sample thickness. Its chromatic aberration correction in IR range is powerful for simultaneous two-wavelength excitation imaging.
- Working distance: 2.0 mm
- Numerical aperture: 1.10
- Chromatic aberration correction: from visible to 1300nm
- Nano Crystal Coat applied.
CFI Apochromat LWD Lambda S 20XC WI
This highly versatile objective provides superior performance thanks to its high numerical aperture, wide field of view and long working distance.
- Working distance: 0.95 mm
- Numerical aperture: 0.95
- Chromatic aberration correction: from visible to infrared.
- Nano Crystal Coat applied.
CFI Apochromat Lambda S 40XC WI
This high NA water immersion objective provides brighter and higher resolution images and is ideal for confocal live cell imaging.
- Numerical aperture: 1.25
- Chromatic aberration correction: from UV through to near IR
- Nano Crystal Coat applied.
CFI Plan Apochromat IR 60XC WI
This lens has the industry’s highest NA for a 60X water immersion objective lens, maintaining flatness throughout the field of view while showing good correction of chromatic aberration over a wide range of wavelengths.
- Numerical aperture: 1.27
- Super image flatness
- Chromatic aberration correction: from visible to infrared.
- Nano Crystal Coat applied.
Nano Crystal Coat for superior transmission
Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that lowers the reflectance index, facilitating the passage of light through the lens rather than reflecting it, and thus providing superior light transmission.
Objectives for multiphoton microscopy
| CFI90 20XC Glyc*2 | NA 1.00 | WD 8.20 | Nano Crystal Coat |
|---|---|---|---|
| CFI Plan Apochromat 10XC Glyc | NA 0.50 | WD 5.50 | Nano Crystal Coat |
| CFI75 Apochromat 25XC W 1300 | NA 1.10 | WD 2.00 | Nano Crystal Coat |
| CFI75 Apochromat 25XC W | NA 1.10 | WD 2.00 | Nano Crystal Coat |
| CFI75 LWD 16X W | NA 0.80 | WD 3.00 | |
| CFI Apochromat NIR 40X W | NA 0.80 | WD 3.50 | |
| CFI Apochromat LWD Lambda S 40XC WI | NA 1.15 | WD 0.62-0.58 | Nano Crystal Coat |
| CFI Apochromat LWD Lambda S 20XC WI | NA 0.95 | WD 0.95 | Nano Crystal Coat |
| CFI Apochromat Lambda S 40XC WI | NA 1.25 | WD 0.20-0.16 | Nano Crystal Coat |
| CFI Plan Fluor 20XC MI | NA 0.75 | WD 0.51-0.35 (Oil) 0.51-0.34 (Water) 0.49-0.33 (glycerin) | |
| CFI Plan Apochromat IR 60XC WI | NA 1.27 | WD 0.18-0.16 | Nano Crystal Coat |
| CFI Plan Apochromat VC 60X WI | NA 1.20 | WD 0.31-0.28 | |
| CFI75 Apochromat LWD 20XC W | NA 1.00 | WD 2.8 | Nano Crystal Coat |
*1: Some tissue clearing reagents may damage these products. Please confirm with a distributor before purchasing the product.
*2: Compatible with Ni-E/FN1 microscopes with a dedicated nosepiece
Product Brochure
Recommended Model:
AX R MP with NSPARC
The newest multiphoton microscope from Nikon is a top choice for deep imaging. The next generation 2K resonant scanner captures fast in vivo dynamics with high resolution over a large field of view. The AX R MP also supports confocal imaging with visible wavelengths and super-resolution using the NSPARC detector.