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University of Southern Denmark

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Center of Excellence

University of Southern Denmark

Nikon and the Danish Molecular Biomedical Imaging Centre at Southern Denmark University have partnered to create the Nikon Center of Excellence for Molecular Biomedical Imaging.

The Nikon Center of Excellence for Molecular Biomedical Imaging provides bioimaging infrastructure and services to research groups at the University of Southern Denmark as well as to users from other Danish universities and companies. Thanks to a dedicated bioimaging research environment, well-trained team and national, and international collaborations, the center supports high-level research as well as education and training.

Research at the center is focused on using advanced bioimaging techniques to help understand and develop treatments for human diseases such as diabetes and cancer. For example, using Stochastic Optical Reconstruction Microscopy (Nikon N-STORM) we can study spatially resolved transcriptomics at a subcellular level in cells or tissue samples.

The Center offers a wide range of normal imaging modalities from automated widefield epifluorescent microscopes to spinning-disk and point scanning confocal. We also offer several supper resolution techniques including:

  • Structured Illumination Microscopy (Nikon N-SIM) that permits, multicolor live-cell imaging with a typical lateral resolution of 120 nm.
  • Stochastic Optical Reconstruction Microscopy (Nikon N-STORM) that resolves structures down to 20 nm.

Furthermore, the center provides access and training for imaging analysis using NIS-Elements.

Contact

Academic Director

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Facility Manager

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Address

Danish Molecular Biomedical Imaging Center (DaMBIC)
5230 Odense M, Campusvej 55
Office number: V12-604b-1


Systems Available

ECLIPSE Ti2-E Widefield Inverted Microscope

The ECLIPSE Ti2-E Inverted research microscope features a groundbreaking 25 mm field of view, maximizing the sensor area of large-format CMOS cameras and significantly improving data throughput. The Ti2-E's unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2-E's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging.

AX R MP with ECLIPSE Ti2

Configured with Nikon's powerful ECLIPSE Ti2 inverted microscope, the AX R MP multiphoton features a high-speed resonant scanner with 2K resolution. The system can capture in a single scan dynamics that span a wide area with superior spatial and temporal resolution. 

ECLIPSE Ti2 with CrestOptics DeepSIM

DeepSIM super-resolution technology is based on multi-spot lattice structured illumination and provides a reliable and affordable solution to study subcellular structures with an XY resolution of 100 nm. The ECLIPSE Ti2 / DeepSIM super-resolution system is easy-to-use and enables scientists to access deep data from their biological samples without requiring any special sample preparation or dyes.

N-SIM S with ECLIPSE Ti2-E

The N-SIM S Super Resolution Microscope is a unique high-speed structured illumination system that achieves acquisition speeds of up to 15 fps, enabling fast biological processes to be captured at twice the spatial resolution of conventional light microscopes (up to 115nm in XY). Combining the N-SIM S and ECLIPSE Ti2-E microscope gives you the flexibility to select a location in the confocal image and switch to super-resolution to view the desired part of the location in minute detail.

N-STORM

N-STORM is a super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology (licensed from Harvard University) and Nikon's ECLIPSE Ti research inverted microscope. The N-STORM super-resolution microscope provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes.

A1R Confocal with ECLIPSE Ti-E Inverted Microscope

Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon's A1R is a powerful tool for the imaging and visualization of intracellular dynamics and interaction. The system comes equipped with a unique hybrid scan head incorporating both an ultra high-speed resonant scanner and a high-resolution galvano scanner, enabling simultaneous bleaching/ photoactivation and acquisition.

ECLIPSE Ti-E Yokogawa CSU-X1 Spinning Disc Confocal

The CSU-X1 field scanning confocal spinning disk system from Yokogawa Life Sciences, integrated with Nikon’s ECLIPSE Ti-E inverted microscope, allows users a flexible and powerful system for a wide range of imaging applications. Field scanners are renowned for their low dosage, specimen-friendly characteristics, making them ideal for live cell or organism applications.