Center of Excellence

Princeton University

Princeton’s Nikon Center of Excellence was established to provide cutting-edge imaging technology to the local scientific community and to establish a mechanism for the exchange of ideas, methodologies and technologies between Princeton’s investigators and Nikon’s technology development teams.

The Center enables users to investigate the dynamics of single molecules to whole organisms and tissues by providing state-of-the art confocal and super-resolution imaging systems and expert technical guidance and support from the Center’s Director.

Contact

CofE Director

email hidden; JavaScript is required

Address

Princeton University
Department of Molecular Biology
116A Moffett Laboratory
Washington Road,
Princeton, NJ 08544
(609) 258-5432

Website

Princeton University

Systems Available

N-STORM/TIRF/CSU-21 Super-Resolution & Spinning Disk Confocal System

This system combines super-resolution N-STORM single molecule imaging with spinning disk confocal microscopy and ablation to provide a multimodal instrument suited for many biological imaging applications.

Components

ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
N-STORM super-resolution single molecule localization microscopy system
4-line laser unit (405nm, 488nm, 561nm, 647nm)
Andor iXon 897 EMCCD camera
Mad City Labs Nano-Z series Z-axis piezo stage
Okolab stage-top incubation system
Andor Zyla 4.2 sCMOS camera
Rapp OptoElectronic UGA Firefly Ablation Module
Yokogawa CSU-21 spinning disk confocal
NIS-Elements software

CSU-21 Spinning Disk Confocal System

Fast multichannel confocal imaging is realized by combining spinning disk confocal scanner with emission splitter, with stimulation additionally provided using a galvo-based system.

Components

ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
Yokogawa CSU-21 spinning disk confocal
Andor TuCam two-camera adapter for simultaneous two-color imaging
Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
Hamamatsu ImagEM EMCCD camera
Large format DS-Ri2 color CMOS camera
Mad City Labs Nano-Z series Z-axis piezo stage
Okolab stage-top incubation system
4-line laser unit (405nm, 488nm, 561nm, 640nm)
Bruker Mini-Scanner galvo-based stimulation system
Anton-paar 502 Rheometer
NIS-Elements software

N-SIM Super-Resolution System

This N-SIM structured illumination super-resolution system beats the diffraction limit of resolution by a factor of two and features emission splitter for simultaneous two-color imaging.

Components

ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
N-SIM super-resolution structured illumination microscopy system
Two-camera emission splitter for simultaneous 2-color imaging
2x Andor iXon 897 EMCCD camera
Tokai Hit stage-top incubation system
NIS-Elements software

A1Rsi Resonant-Scanning Confocal System

This A1R HD resonant scanning confocal system features both a spectral detector for linear unmixing of multiple sisgnals and DMD for patterned stimulation, perfect for applications such as optogenetics.

Components

ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
A1R HD high-definition resonant scanning confocal system
A1-DUS 32-channel spectral detector
LUN-4 4-line laser unit (405 nm, 488 nm, 561 nm, 640 nm)
DU4G GaAsP 4-channel confocal detector
Mad City Labs Nano-Z series Z-axis piezo stage
Warner Instruments incubation system
Mightex Polygon400 DMD for patterned illumination (Lumencor AURA II light engine)
Andor Zyla 4.2 sCMOS camera
NIS-Elements software