BioPipeline Slide 8

Microscope base

Nikon Ni-E motorized upright microscope

System type

Microscope-based semi-automated whole slide imaging system

Primary application

Medium-throughput whole slide imaging (WSI); also supports routine research microscopy

Configurations

Brightfield, Epifluorescence, or Brightfield and Epifluorescence

Antivibration table

Recommended, especially for 20x/40x magnfication imaging to reduce environmental vibration

Imaging modalities

Transmitted light brightfield and brightfield contrasting techniques (phase, darkfield, DIC); widefield epifluorescence

Illumination

Up to 8-channel epifluorescence LED; LED transmitted light

Objectives

All air objective lenses compatible; selectable per configuration

Autofocus

AI-assisted; automatic mult-focus surface map for uneven samples

Imaging methods

Multidimensional XY, Z, wavelength, multi-stage position including tiling

Hardware triggering

Enabled for continous acquisition during XYZ movement

Sensor type

CMOS

Shutter type

Global shutter

Resolution

5 MP (2448 × 2048 px)

Pixel size

3.45 μm × 3.45 μm

Interface

USB 3.0

Standard slide capacity

8 slides per run

Optional stage insert

4-slide insert for 2×3" / 52×76 mm slides

Focus surface mapping

Various modes and sampling frequency according to the sample flatness

10 x 10mm Scan Speed

Continuous scanning acquires 10x10mm in 60 seconds @10x; tiling and blending occurs in parallel with the acquisition of the next slide.

Extended Depth of Focus (EDF) result images are acquired

Z stack capability

Software platform

NIS-Elements AR with dedicated slide scanning module

Acquisition interface

Guided wizard for brightfield and fluorescence; suitable for novice users

Sample detection

AI-assisted automatic sample selection with user interaction

Annotation

Project and slide annotation; multiple ROI support

Analysis modules

Optional NIS-Elements GA3; NIS-CC; additional modules selectable

Data sharing

Unified acquisition and analysis across all Nikon microscope platforms

Optional storage

BioPipeline Server: 10GbE dedicated network, 30× workstation capacity

File Formats

Nikon ND2, OME-TIFF, JPEG

Barcodes

1D: Codabar, Code39, Code93, Code128, EAN8, EAN13, ITF, DataBar, DataBarExpanded, UPCA, UPCE2D: Aztec, DataMatrix, MaxiCode, PDF417, QRCode, MicroQRCode, RMQRCode

Dimensions (W × D × H, without detector)

32 cm × 60 cm × 510 cm typical; +70 mm H with additional EPI port

Weight (approx)

32 kg

Power consumption

140 W (microscope body only)