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Multiphoton Microscope System

Look Deeper

- Explore the hidden depths of whole organisms in panoramic views -

Nikon’s multiphoton confocal microscopes, which clearly visualize minute structures deep within living organisms, have evolved even further. In a single scan, the AX R MP can capture dynamics that span a wide area with superior spatial and temporal resolution.

In addition, the space available for samples has been greatly expanded to provide the flexibility to observe biological samples in their natural postures. The AX R MP offers a large field of view with high-speed, high resolution scanning in a single unit.

For broad research areas

Download Multiphoton Brochure (1.49MB)

Key Features

Imaging of a large region using a high-speed resonant scanner with 2K resolution

Blood vessels (Alexa 488) and epidermis (SHG) in a mouse paw
Using CFI75 Apochromat LWD 20XC W (optional)

Capture more data than ever before

Featuring a field-of-view with a diagonal of 22 mm for both resonant and galvano scanners, the AX R MP captures more data per single frame.

High-speed scanning that reliably captures dynamic events

The resonant scanner supports ultra-high-speed imaging at 720 fps (512 x 16 pixels). It can capture the rapid dynamics of living organisms with reduced phototoxicity.

High resolution for visualizing minute structures

The galvano scanner enables 8k x 8k high resolution images to be obtained, with a pixel density that allows Nyquist sampling at any magnification. The high-speed resonant scanner supports high resolution imaging with pixel densities up to 2k x 2k. Both can visualize morphological changes in deeper regions in fine detail.

Extreme flexibility in specimen preparation

The space under the objective is 40 cm high, allowing versatile specimen studies. The variable angle of the objective makes specimens highly accessible.

Large free space around the sample

With even more space under the objectives, the new stands accompanying the AX R MP support the very large experimental apparatus needed for in vivo live imaging of entire animals. Two different stands are available to accommodate different specimen observations.

With gate stand

With single stand

Observe samples in their natural postures

Nikon has developed a new CFI75 single tilting nosepiece that can adjust the objective to different angles. It enables observation of a sample in the lateral and oblique directions without changing its orientation, reducing the load on the sample. The Piezo Z device (optional) allows for highly accurate, high-speed Z imaging.

Vertical rotation (±90°)

Horizontal rotation (±100°)

Z axis coarse movement (±3 mm)
Piezo Z device stroke: 400 μm

Objective optimized for deep imaging providing bright images over the entire field of view

Nikon offers a full lineup of high NA objectives for multiphoton imaging that provide chromatic aberration correction up to the near-infrared range. The newly developed CFI75 Apochromat LWD 20XC W supports large FOV imaging of 22 mm. It provides bright images even at the edge of its large field of view at low magnifications. It achieves both a high NA (1.00) and a long working distance (2.8 mm), enabling crisp imaging deep within the sample.

CFI75 Apochromat LWD 20XC W
(NA: 1.00, WD: 2.8 mm)

CFI90 20XC Glyc
(NA: 1.00, WD: 8.20 mm)

CFI75 Apochromat 25XC W 1300
(NA: 1.10, WD: 2.00 mm)

CFI Apochromat Lambda S 40XC WI
(NA: 1.25, WD: 0.20-0.16 mm)

CFI Plan Apochromat 10XC Glyc
(NA: 0.50, WD: 5.50 mm with upright microscope)

CFI Apochromat LWD Lambda S 20XC WI
(NA: 0.95, WD: 0.95 mm)

High-sensitivity detection of signals from deep areas

4-channel episcopic GaAsP NDD

Non-descanned detectors (NDDs) and optical couplings are designed with efficiency as a key requirement, placed close to the objective lens back aperture and implemented with low noise electronics.