Objective lenses designed to provide the highest quality point spread functions for super-resolution imaging.
Aligned and inspected using wavefront aberration measurement technologies to ensure the lowest possible asymmetric aberration and superior optical performance required for super-resolution imaging.
Compatible with the ultrahigh power lasers required to induce rapid photoswitching of fluorophores under N-STORM imaging. They provide improved axial chromatic aberration correction to achieve the highest level of precision in localization and image alignment for 3D multi-color STORM imaging.
Silicone immersion objectives
The CFI SR HP Plan Apochromat Lambda S 100XC Sil uses high viscosity silicone oil with a refractive index close to that of live cells as an immersion liquid. Because of this improved refractive index compatibility, these objectives can provide improved photon collection capability and resolution when performing super-resolution imaging deeper inside a specimen. They provide superior chromatic aberration correction and high transmittance over a broad range of wavelengths.
The N-SIM S/N-SIM E is compatible with the CFI Plan Apochromat Lambda 60XC and CFI Plan Apochromat Lambda 40XC. These low-magnification, wide field-of-view dry objectives enable both super-resolution imaging and confocal imaging without switching lenses.
AC type models
The CFI SR HP Apochromat TIRF 100XAC Oil and CFI SR Plan Apochromat IR 60XAC WI are compatible with the Auto Correction Collar of the Ti2-E inverted microscope, which enables highly precise and easy adjustment of the correction collar.
|Cover glass thickness||Correction ring||Observation|
|CFI SR HP Plan Apochromat Lambda S 100XC Sil||Diagram||Graph||1.35||0.30 (0.31-0.29): 23˚C, 0.29 (0.30-0.28): 37˚C||0.15-0.19 (23-37℃)||V||BF, DIC, POL, FL (Visible light, UV)|
|CFI SR HP Apochromat TIRF 100XC Oil||Diagram||Graph||1.49||0.12 (0.16-0.10): 23˚C, 0.12 (0.15-0.09): 37˚C||0.13-0.19 (23℃)|
|V||BF, DIC, PH**, POL, FL (visible light, UV*)|
|CFI SR HP Apochromat TIRF 100XAC Oil||Diagram||Graph||1.49||0.12 (0.16-0.10): 23˚C, 0.12 (0.15-0.09): 37˚C||0.13-0.19 (23℃)|
|V(Auto)||BF, DIC, PH**, POL, FL (visible light, UV*)|
|CFI HP Plan Apochromat VC 100X Oil||Diagram||Graph||1.40||0.13||0.17||BF, DIC, PH**, POL, FL (visible light, UV*)|
|CFI SR Plan Apochromat IR 60XC WI||Diagram||Graph||1.27||0.17 (0.18-0.16)||0.15-0.19||V||BF, DIC, PH**, POL, FL (visible light, UV, NIR)|
|CFI SR Plan Apochromat IR 60XAC WI||Diagram||Graph||1.27||0.17 (0.18-0.16)||0.15-0.19||V(Auto)||BF, DIC, PH**, POL, FL (visible light, UV, NIR)|
|CFI Plan Apochromat Lambda 40XC||Diagram||Graph||0.95||0.21 (0.25-0.16)||0.11-0.23||V||BF, DF (Oil), DIC, POL, FL (visible light, UV*, NIR)|
|CFI Plan Apochromat Lambda 60XC||Diagram||Graph||0.95||0.15 (0.21-0.11)||0.11-0.23||V||BF, DF (Oil), DIC, POL, FL (visible light, UV*, NIR)|
PH: Phase contrast
POL: Simple polarizing
*Possible but not recommended
**External phase contrast observation is possible with Eclipse Ti2-E