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S Plan Fluor LWD Series

High-resolution, large-FOV objectives that are suitable for observation using a plastic cell-culture dish

CFI S Plan Fluor LWD 20XC and CFI S Plan Fluor LWD ADM 20XC objectives for biological microscopes are effective in research utilizing stem cells and drug design research. They are capable of a long working distance*1 and provide a high numerical aperture. These objectives provide clear images even in observation with plastic cell-culture dishes, which are frequently used in various types of research.

The CFI S Plan Fluor LWD 20XC is suitable for brightfield, fluorescence and DIC observation, and the CFI S Plan Fluor LWD ADM 20XC can be used for brightfield, fluorescence and phase contrast observation.


*1 Working distance: The distance from the tip of the objective lens to the observation target when the target is in focus. A long working distance has numerous advantages, such as prevention of contact with the sample when changing objectives.

다운로드 Objectives Brochure (8.75MB)


Cover glass thicknessCorrection ringObservation
CFI S Plan Fluor LWD 20XCDiagramgraph0.701.3-2.30-1.8BF, DF (Dry/Oil), DIC, POL, FL (visible light, UV)
CFI S Plan Fluor LWD ADM 20XCDiagramgraph0.701.3-2.30-1.8BF, DF (Dry/Oil), PH, FL (visible light, UV)

BF: Brightfield
DF: Darkfield
PH: Phase contrast
POL: Simple polarizing
FL: Fluorescence

*Possible but not recommended
**External phase contrast observation is possible with Eclipse Ti2-E

주요 특징들

Long working distances and high NA

The new objectives provide long working distances of 1.3 mm to 2.3 mm, which are compatible with observations using thick-bottomed plastic dishes and observations of thick observation targets. In addition, the high NA of 0.70 enables rapid acquisition of sharp images even during fluorescence observation, which requires bright images.

25 mm FOV supports wide visual field observation

The new objectives provide 20X observations with an FOV of 25 mm*2 when used together with the ECLIPSE Ti2 Inverted Research Microscope. They allow effective observation of wide areas and rapid acquisition of large amounts of observation data, enabling high cell screening and image stitching*3 throughput.

*2 FOV (field of view: the diameter of the observable image in a microscope)

*3 Image stitching: an image processing method that combines neighboring images to create one large

Fluorescence images of the actin, nuclei and mitochondria of HeLa cells are simultaneously acquired and then overlaid. Acquired with the CFI S Plan Fluor LWD 20XC objective mounted on the Confocal Microscope A1+.

An overall image of HeLa cells (acquired by phase contrast observation) and an image of nuclei and mitochondria (simultaneously acquired by fluorescence observation) are overlaid. Acquired with the CFI S Plan Fluor LWD ADM 20XC objective mounted on the Inverted Research Microscope Ti2-E.