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CFI75 Water Dipping Series

High numerical aperture, long working distance lenses for multiphoton imaging and electrophysiology applications


The CFI75 LWD 16X W is a water-immersion objective lens with low magnification, high NA and long working distance. This lens, when combined with Nikon's ECLIPSE FN1 microscope and dedicated magnification module, provides 5.6x, 32x, and 64x magnifications. Because it allows observation from a low magnification wide field to a high magnification, high resolution field with a single objective, the lens is ideal for patch-clamp experiments. With excellent IR transmission, this lens is also suitable for IR-DIC observation and the objective's high NA provides superb image quality in combination with confocal laser microscopes. 
In addition, the 2mm field of view (magnification 5.6x) and wide 45° approach angle make manipulator control and positioning extremely easy.

CFI75 Apochromat 25XC W

This lens provides a high numerical aperture of 1.10 while still maintaining a long working distance of 2.0mm. Chromatic aberration is well-corrected up to the near-infrared range. This lens features an adjustable ring to correct for depth-dependent spherical aberration. Together with its 33° manipulator pipette access angle, it is ideal for deep imaging of live specimens using multi-photon excitation and physiology research applications.

다운로드 Objectives Brochure (8.74MB)


Cover glass thicknessCorrection ringObservation
CFI75 Apochromat LWD 20XC WDiagramGraph1.002.800BF, FL (visible light, NIR)
CFI75 Apochromat 25XC WDiagramGraph1.102.000BF, DF (Oil), DIC, NIR DIC, POL, FL (visible light, UV)
CFI75 Apochromat 25XC W 1300DiagramGraph1.102.000BF, DF (Oil), DIC, NIR DIC, POL, FL (visible light, UV)
CFI75 LWD 16X WDiagramGraph0.803.000BF, DF (Oil), DIC, NIR DIC, POL, FL (visible light, UV)

BF: Brightfield
DF: Darkfield
PH: Phase contrast
POL: Simple polarizing
FL: Fluorescence

*Possible but not recommended

**External phase contrast observation is possible with ECLIPSE Ti2-E