A personal super-resolution microscope that provides the same high resolution as the N-SIM S.
N-SIM E is a streamlined, affordable super-resolution system that provides double the resolution of conventional light microscopes. Combining N-SIM E and a confocal microscope allows you the flexibility to select a location in the confocal image, and easily switch to view it in super-resolution, enabling the acquisition of more detail. Voyaging into the nanoverse opens up the possibilities of this new frontier in life sciences.
Double the resolution of conventional light microscopes
The N-SIM E utilizes Nikon's innovative approach to "structured illumination microscopy" technology. By pairing this powerful technology with Nikon’s renowned objectives with unparalleled NA of 1.49, the N-SIM E nearly doubles the spatial resolution of conventional light microscopes (to approximately 115 nm*), and enables detailed visualization of the minute intracellular structures and their interactive functions.
* This value is measured FWHM of 100 nm beads exited with 488 nm laser in 3D-SIM mode.
Fast 1 sec/frame temporal resolution for super resolution imaging
N-SIM E provides fast imaging performance for Structured Illumination techniques, with a time resolution of approximately 1 sec/frame, which is effective for live-cell imaging.
Acquire larger fields of view
N-SIM E can acquire super-resolution images with a large field of view of 66 µm square. This larger imaging area enables very high throughput for applications/samples that benefit from larger fields of view, such as a neurons, reducing the amount of time and effort required to obtain data.
Axial super-high resolution imaging with 3D-SIM mode
The 3D-SIM mode generates structured illumination patterns in three dimensions to deliver a two-fold improvement in lateral and axial resolutions. Two reconstruction methods (“slice” and “stack”) are available to optimize results according to application requirements (e.g. sample thickness, speed, etc.). Slice reconstruction is suitable for imaging living cells at specific depths, as it allows axial super-resolution imaging with optical sectioning at 300 nm resolution. Optional stack reconstruction, based on Gustafsson’s theory, is suitable for acquisition of volume data as it can image thicker specimens with higher contrast than slice reconstruction.
Seamless switching between imaging modalities for multi-scale experiments
The N-SIM E can be simultaneously combined with a confocal microscope such as the A1+ or C2+. A desired location in a sample can be specified in a low-magnification/large FOV confocal image and acquired in super-resolution by simply switching the imaging method. Combining a confocal microscope with a super-resolution system can provide a method for gaining larger contextual views of super-resolution information.
3-color multi-laser super-resolution capability
The compact LU-N3-SIM laser unit dedicated for N-SIM E is installed with the three most commonly used wavelength lasers (488/561/640), enabling super-high resolution imaging in multiple colors. It enables the study of dynamic interactions of multiple proteins of interest at the molecular level.
Objectives for super-resolution microscopes
Silicone immersion objectives
Silicone immersion objectives use high viscosity silicone oil with a refractive index close to that of live cells as an immersion liquid. Because of this improved refractive index compatibility, these objectives can provide improved photon collection capability and resolution when performing super-resolution imaging deeper into the specimen. They exhibit superior chromatic aberration correction and high transmittance over a broad range of wavelengths.
The system can be configured with either a 100X oil immersion type, which is suitable for the imaging of fixed samples, or a 60X water immersion type, which is optimal for time-lapse live-cell imaging. The SR objectives are aligned and inspected using wavefront aberration measurement technologies to ensure the lowest possible asymmetric aberration and superb optical performance required for super-resolution imaging.
The N-SIM E is compatible with dry objectives, making both super-resolution imaging and confocal imaging available without switching lenses. Low-magnification, wide field-of-view dry lenses enable high resolution observation even at the periphery of sample tissues.
* Dry objectives support slice reconstruction