In this webinar, Prof. Jonathan Brewer presented the full pipeline, from sample prep and probe design to iterative labelling, 3D imaging with the ECLIPSE Ti2 inverted microscope paired with the Crest X-Light V3 spinning disk confocal, and downstream analysis. Integrated with NIS-Elements JOBS, the workflow delivers robust, user-friendly automation. Biological examples, including melanoma skin models, show how spatial omics can become routine and scalable.