Application Notes

Apoptosis assay using AI-driven fully automated ECLIPSE Ji

September 2023

ECLIPSE Ji with Smart Experiment software enables seamless run from image acquisition to analysis and graph creation. Pretrained Artificial Intelligence (AI) and pre-defined imaging processes automatically optimize image acquisition and analysis condition settings, providing visualized data and EC50 information with simple operation. Apoptosis is a type of programmed cell death. Abnormalities in apoptosis cause various diseases such as cancer, autoimmune diseases, and neurological disorders. Many cancer cells acquire resistance to apoptosis, avoiding cell death and continuing to proliferate. Apoptotic cells can be detected by fluorescence dependent on the activity of Caspase-3/7, a marker for apoptosis. This application note introduces an example of using the Apoptosis module of Smart Experiment to visualize the dose-dependent induction of apoptosis by staurosporine in cancer cells and quantify the effect of the drug by calculating the EC50.