Application Notes
Mitochondrial toxicity analysis using the AI-driven, fully automated smart imaging system ECLIPSE Ji
January 2024
Mitochondria are important organelles that produce energy, and mitochondrial dysfunction is known to be involved in metabolic abnormalities and the development of various diseases. Mitochondrial membrane potential-dependent fluorescent dyes provides unique insights related to mitochondrial health. The ECLIPSE Ji digital inverted microscope equipped with NIS-Elements Smart Experiment (SE) software enables automated, seamless imaging workflows from acquisition to analysis and data visualization. This application note introduces the Mitochondrial Toxicity SE module, which was used to analyze drug-induced mitochondrial toxicity by measuring the fluorescence intensity of a dye that stains healthy mitochondria.