Application Notes
Analysis of DNA Damage using AI-driven fully automated ECLIPSE Ji
September 2023
ECLIPSE Ji with Smart Experiment software enables seamless operation from image acquisition to analysis and graph creation. Pre-trained Artificial Intelligence (AI) and pre-defined imaging procedures automatically optimize image acquisition and analysis condition settings, providing visualized data in a simple, streamlined process. γH2AX is a marker of DNA damage and DNA repair. It is known that histone protein H2AX is phosphorylated to form γH2AX at the site of DNA double-strand break (DSB), which is involved in DNA repair. This application note introduces an example of using the DNA Damage - gammaH2AX module of Smart Experiment to quantify DNA damage by measuring fluorescence intensity derived from immunofluorescence-stained gammaH2AX in the nucleus region.