A1 MP+ / A1R MP+ Multiphoton
High Definition Multiphoton
Discontinued Replaced by AX R MP with NSPARC
Specifications
| A1 MP+ | A1R MP HD | |
|---|---|---|
| Scan head input/output port | 3 laser input ports, 3 signal output ports for standard, spectral and optional detector*1 | |
| FOV | Square inscribed in a Φ18 mm circle | |
| Image bit depth | 4096 gray intensity levels (12 bit) | |
| Pinhole | 12 – 256 μm variable (1st image plane) | |
| Compatible microscopes | ECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type) | |
| Z step | Ti2-E: 0.02 µm, FN1 stepping motor: 0.05 µm, Ni-E: 0.025 µm | |
| Option | Motorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E) | |
| Laser for multiphoton microscopy | A1 MP+ | A1R MP HD |
|---|---|---|
| Compatible laser | Mai Tai HP/eHP DeepSee, InSight DS+*2, InSight DS+ Dual Option*3, InSight X3*2, InSight X3 Dual Option*3 (Spectra-Physics), Chameleon Vision II, Chameleon Discovery*3 (Coherent) | |
| Modulation | Method: AOM (Acousto-Optic Modulator) device Control: power control, return mask, ROI exposure control | |
| Incident optics | 700 - 1080 nm*4,*5, 700 - 1300 nm*2,*3, auto alignment | |
| Laser for confocal microscopy (option) | A1 MP+ | A1R MP HD |
|---|---|---|
| LU-N3 3 laser unit | 405 nm, 488 nm, 561nm lasers are installed; built-in AOTF (Cannot used with A1-DUS spectral detector) | |
| LU-N4/LU-N4S 4laser unit | 405 nm, 488 nm, 561 nm, 640 nm lasers are installed; built-in AOTF (LU-N4 cannot be used with A1-DUS spectral detector) | |
| LU-NV Laser unit | Compatible laser: 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm; built-in AOTF | |
| NDD for multiphoton microscopy | A1 MP+ | A1R MP HD |
|---|---|---|
| Type | Compatible with 1080 nm: Episcopic GaAsP NDD (for Ti2-E/Ni-E/FN1), Diascopic GaAsP NDD (for Ni-E/FN1); Detectable wavelength range 380 - 650 nm*6 Compatible with 1300 nm: Episcopic GaAsP NDD (for Ti2-E/Ni-E/FN1); Detectable wavelength range 380 - 750 nm | |
| Detector | 4 PMTs (3 GaAsP PMTs and 1 Multi-Alkali PMT) | |
| Filter cube | 450/50, 492, 525/50, 575/25, 610/75, 629/53 | |
| Standard fluorescence detector (option) | A1 MP+ | A1R MP HD |
|---|---|---|
| Detectable wavelength range | 400 - 750 nm (400 - 650 nm when using IR laser) | |
| Detector | A1-DU4-2 4 Detector Unit: 4 Multi-Alkali PMTs A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs | |
| Filter cube | 6 filter cubes commonly used for a microscope mountable on each of three filter wheels Recommended wavelengths for multiphoton/confocal observation: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 594LP, 595/50, 700/75 | |
| Diascopic detector (option) | A1 MP+ | A1R MP HD |
|---|---|---|
| Detectable wavelength range | 440 – 645 nm | |
| Detector | Multi-Alkali PMT | |
| Scan head | A1 MP+ | A1R MP HD |
|---|---|---|
| Type | A1-SHSM-2 | A1-SHRM-C |
| Standard image acquisition | Scanner: galvano scanner x2 Pixel size: max. 4096 x 4096 pixels Scanning speed: Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction) Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction) *7 Zoom: 1-1000x continuously variable Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z | |
| High-speed image acquisition | — | Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis) Pixel size: max. 1024 x 1024 pixels Scanning speed: 15 fps (1024 x 1024 pixels), 30 fps (512 x 512 pixels), 60 fps (256 x 256 pixels), 420 fps (512 x 32 pixels), to 720 fps (512 x 16 pixels), 15,600 lines/sec (line speed) Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x) Scan mode: X-Y, X-T, X-Z Acquisition method: High-speed image acquisition, Simultaneous photoactivation and image acquisition |
| IR laser wavelength range | 700 - 1080 nm*4,*5, 700 - 1300 nm*2,*3 | |
| Dichroic mirror | Low-angle incidence method Position: 8 Standard filter: 405/488, 405/488/561, 400-457/514/IR, 405/488/543/638, IR total reflection*2,*3, BS20/80, IR | |
| Simultaneous stimulation option | — | Optional hyper selector Visible Stimulation/Visible imaging: Stimulation by 405nm, Imaging by 488-750nm Visible stimulation/IR imaging: Stimulation by 405nm, 488nm or 561nm (selectable by NDD dichroic mirror), Imaging by 800nm-1080nm (1080nm configuration), 820nm-1080nm (1300nm configuration) Optional 1st dichroic mirror :High reflection mirror 405,488,561,800-1080 |
| Spectral detector (option) | A1 MP+ | A1R MP HD |
|---|---|---|
| A1-DUS spectral detector unit | Number of channels: 32 Wavelength detection range: 400 - 750 nm Spectral image acquisition speed: 4 fps (256 x 256 pixels) Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode) Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps Compatible with galvano scanner only | |
| A1-DUVB-2 GaAsP detector unit | Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest:320 nm Maximum pixel size: 4096 x 4096 (CB mode/VB mode) Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps Compatible with galvano and resonant scanners | |
| Software | A1 MP+ | A1R MP HD |
|---|---|---|
| Display/image generation | 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing | |
| Image format | JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS | |
| Application | FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization | |
| Control computer | A1 MP+ | A1R MP HD |
|---|---|---|
| OS | Windows 10 Pro 64bit, English version or Japanese version OS Version 1704 Windows 7 Professional, 64bit, SP1 English version or Japanese version Windows Update KB3118401 or later | |
| CPU | Intel Xeon E5-2643v4 (3.40GHz, 6 cores, 20MB, 2400MHz) or higher | |
| RAM | 16GB, 32GB or 64GB | |
| HDD | 1st HP Z Turbo G2 512GB PCIe M.2 SSD, 2nd SATA 2TB | |
| Optical Drive | Super Multi drive, up to x 16 speed or higher | |
| Graphics | NVIDIA Quadro K620/ K2200/ K4200/ M2000/ M4000/ M5000 (PCI Express / two-screen split display supported) | |
| Extension slot | Two PCI Express 3.0 (x16) slots (one slot to be used for graphics), One PCI Express 3.0 (x8 mechanical, x4 electrical) slot, One PCI Express 2.0 (x8 mechanical, x4 electrical) slot, One PCI Express 2.0 (x1) slot | |
| LAN port | 10/100/1000 Network/Interfacex2 (for connection to controller, for connection to external LAN) | |
| Monitor | 1600 x 1200 or higher resolution, dual monitor configuration recommended | |
*1 FCS/FCCS/FLIM is possible in combination with third-party systems.
*2 When using accessories with 1300 nm.
*3 When using accessories with 1300 nm and Dual IR.
*4 When using accessories with 1080 nm.
*5 When using accessories with 1080 nm and visible light photoactivation/IR imaging.
*6 400 - 650 nm when using diascopic NDD.
*7 Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.
Product Brochure
Recommended Model:
AX R MP with NSPARC
The newest multiphoton microscope from Nikon is a top choice for deep imaging. The next generation 2K resonant scanner captures fast in vivo dynamics with high resolution over a large field of view. The AX R MP also supports confocal imaging with visible wavelengths and super-resolution using the NSPARC detector.