Inverted Microscope Systems

Application Notes

Application of Patterned Illumination Using a DMD for Optogenetic Control of Signaling

Digital micromirror devices (DMDs) are powerful tools for photostimulation applications, including photoconversion and optogenetic manipulation, owing to their robust ability to produce novel illumination patterns with high spatiotemporal resolution. In this Application Note we showcase recent work describing how DMD technology integrated into a Nikon system can be applied toward light-gated optogenetic control of intracellular signaling.


Hardware Triggering: Maximizing Speed and Efficiency for Live Cell Imaging

Live cell imaging experiments now require higher speeds and more data throughput than ever before. Nikon Instruments has robust tools that enable hardware triggering of imaging devices in microscopy via direct signaling between hardware. This minimizes delays, synchronizes devices, and reduces the exposure of specimens to light. This Application Note explains how Nikon’s NIS-Elements hardware triggering workflow operates, and details its benefits for common time-lapse acquisition routines.


Robotic Microscopy with the Nikon Ti2 for High-Content Analysis Applications

Robotic Microscopy—a combination of high-content screening methods—enables multivariate experimental approaches with large cell populations and member-level sensitivity. Here we explore how the new Nikon Ti2 line of inverted research microscopes is uniquely suited to Robotic Microscopy applications, focusing on work utilizing induced pluripotent stem cells (iPSCs) as disease models in drug screening.


The optimal parameters for ICSI – perfect your ICSI with precise optics

The current prevalence of infertility lasting for at least 12 months is estimated to be around 9% worldwide for couples aged 20-44 [1]. Two main fertilization techniques are used during the process: standard in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). ICSI accounts for 66% of the treatments worldwide and conventional IVF around 33%. ICSI fertilizes 50% to 80% of eggs [2], and the mean pregnancy rate per embryo transfer was 33% after ICSI in Europe in 2014. Although other factors might play a role in a successful pregnancy rate, maximizing ICSI success is the first step towards a successful pregnancy. At the OVA Clinic Zurich, fertilization rates for ICSI are as high as 80-90%. Here we describe how the optimal parameters and the most precise equipment can help to achieve the best ICSI.