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Crest X-Light V2

High‐speed, large field of view spinning disk confocal imaging

The X-Light V2 series of laser-free confocal spinning disk systems from Crest Optics, integrated with Nikon’s superb microscope platforms, delivers high speed imaging and expanded field of view (up to 25 mm) within a budget-friendly solution. With this solution you can upgrade your favourite widefield system into a confocal without compromising speed or field of view.

The fast imaging speed makes the X-Light V2 the perfect instrument for 3D imaging of cells and tissues or for capturing fast dynamics in cell motility, signalling and transport applications. The gentle illumination provided by the LED light source allows long-term time-lapse studies of cell division and gene expression. With the exclusive 25 mm field of view of the X-Light V2 L-FOV, large samples can be captured with minimal scanning and stitching, and often in a single image capture.


Key Features

Large field of view

X-light V2 L-FOV model images more in one capture and increases your system throughput by reducing the need of stitching to visualize your sample or the number of images required to collect statistically significant data.

Mouse kidney imaged, in a single capture, on Ti2 with X-Light V2 L-FOV using a 20x objective and Nikon DS-Qi2 Camera, which sensor is larger than 25 mm


Confocal and widefield imaging on the same instrument

The intelligent design of the X-Light V2 series, together with the user-friendliness of NIS-Elements software, allows for the switch between widefield and confocal imaging in just one click. The camera and other components, such as light source and filters, are shared between both modalities making this solution economic and flexible.

Daphnia water flea imaged on a Ti2 with X-Light V2 L-FOV using 20x objective and Prime 95B 25mm sCMOS camera


Improved image quality and resolution

Latest generation X-light V2 series includes iris diaphragms in both the emission and transmission paths to decrease background by 3-4 folds without affecting signal brightness. NIS-Elements, Nikon's unified software platform, provides an intuitive workflow for confocal imaging. Higher resolution images can be generated with a single click. The software assesses the captured image and automatically determines processing parameters to achieve increased resolution.

Daphnia water flea imaged, in a single capture, on a Ti2 with X-Light V2 L-FOV using 20x objective and Prime 95B 25mm sCMOS camera. Deconvolution applied using NIS-Element