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UT Austin

The Center for Biomedical Research Support Microscopy and Imaging Center is dedicated to supporting the research community at UT Austin and beyond by providing access to state-of-the-art imaging instrumentation, as well as technical assistance and education, to researchers across all disciplines.

Opening: Wednesday, March 6, 2019; From left to right: Andy Davis (General Manager, Sales, Nikon Instruments Inc.), Toshiaki Nagano (President & Chief Executive Officer, Nikon Instruments Inc.), Daniel Jaffe (Vice President for Research, The University of Texas at Austin), Andreas Matouschek (Professor, Core Facilities Director, Molecular Biosciences, Office of the VP for Research, The University of Texas at Austin)

Kontakt

CofE Director

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Address

The University of Texas at Austin
MBB 1.426
2500 Speedway A4800 Avenue
Austin TX 78712

Website

UT Austin



Systems Available

A1R Dual Beam Large Format Multiphoton System

  • Open architecture to accommodate large stereotactic equipment, treadmills and other devices
  • CFI75 Apochromatic 25X W 1.1 N.A. objective for deep imaging
  • Epi-fluorescence for locating samples
  • LU-NV 6-line visible laser unit (405, 445, 488, 515, 561, 640nm)
  • Coherent Chameleon Discover Dual Beam laser unit with wide tuning (680-1300nm)
  • NIS-Elements C software

A1R High-Speed Point Scanning Confocal

  • Ti2-E inverted platform with Perfect Focus System 4 for precise, automatic focus maintenance
  • Motorized, encoded stage for accurate XY positioning
  • DIC and brightfield transmitted options
  • Epifluorescence for locating samples
  • LUN4 4-line visible laser unit
  • NIS-Elements C software

N-STORM/TIRF System

  • Ti2-E inverted platform with Perfect Focus System 4 for precise, automatic focus maintenance
  • Motorized, encoded stage for accurate XY positioning
  • Stage-top piezo for fast Z-stacking
  • Large FOV epi-fluorescence imaging (DAPI, GFP, TxRed)
  • DIC and brightfield transmitted options
  • Motorized H-TIRF with TwinCam for simultaneous, 2-color TIRF
  • N-STORM super-resolution with 10x improvement in spatial resolution
  • 4-line, high-powered LUNV laser unit (405, 488, 561, 640nm) for TIRF and STORM
  • Tokai HIT stage top incubator for live-cell imaging
  • NIS-Elements AR with STORM analysis, triggering, illumination sequence, deconvolution, and JOBS

Spinning Disk Live Cell Imaging System

  • Ti2-E inverted platform with Perfect Focus System 4 for precise, automatic focus maintenance
  • Motorized, encoded stage for accurate XY positioning
  • Stage-top piezo for fast Z-stacking
  • Epi-fluoresence module for widefield imaging and ocular visualization (DAPI, GFP, TxRed, Cy5)
  • DIC and brightfield transmitted options
  • Large field of view camera (Nikon DS-Qi2 monochrome CMOS with 36x23.9mm sensor, F-mount)
  • Yokogawa CSU-W1 with dual camera (Andor iXon Ultra 888 EMCCDs) and 4-line high-powered laser unit (405, 488, 561, 640nm)
  • Tokai HIT STXG stage-top incubator for dishes
  • NIS-Elements AR with triggering and HCA

Nikon High Content Screening System

  • Ti2-E inverted platform with Perfect Focus System 4 for precise, automatic focus maintenance
  • High-speed linear motorized stage (Prior)
  • Epi-fluorescence module for widefield imaging and ocular visualization (DAPI, CFP, GFP, YPF, TRITC, mCherry, Cy5)
  • DIC and brightfield transmitted options
  • Dual camera system for wide-field epi-fluorescence, DIC/brightfield and Image Stitching
  • Well-plate loader with 20 well-plate capacity (fits all standard microtiter plates up to 19mm thick)
  • Piezo Z drive for high-speed z-stacking
  • Oko-Labs incubation system for single well-plates
  • NIS-Elements HCA software with online acquisition and offline analysis

Ni-E Upright Wide Field Fluorescence Microscope System

  • Fully motorized upright microscope, including XY and Z
  • DIC imaging
  • Epifluorescence for wide-field imaging and ocular visualization (DAPI, GFP, mCherry, Cy5)
  • Two camera options for quantitative fluorescence and color-imaging (Andor Zyla 4.2 Plus, Nikon Fi3 color CMOS camera)
  • NIS-Elements AR

Nikon SMZ25 Motorized Stereo Microscope System

  • Auto Link Zoom (ALZ) for automatic adjustment of zoom factor when switching objective lenses (to maintain the same field of view)
  • 25:1 zoom ratio with high N.A.
  • Intelligent dual objective nosepiece for accurate meta-data
  • LED based illumination systems for Diascopic and Reflected light imaging
  • Fluorescence imaging (DAPI, CFP, GFP (bandpass and longpass), YPF, mCherry, Cy5)
  • Nikon Fi3 color CMOS camera
  • NIS-Elements AR software with Extended Depth of Focus (EDF)

Ts2R Inverted Wide Field Fluorescence Microscope System

  • Compact inverted research microscope
  • Phase Contrast imaging
  • Epi-fluorescence imaging with high-speed switching (DAPI, CFP, GFP, YFP, TRITC, mCherry, Cy5)
  • Motorized XY and Z
  • NIS-Elements AR software