iLas2

Multimodal illumination system for Ti2-LAPP

iLas2 for Ti2 integrates TIRF, photostimulation, and epifluorescence modalities through a single episcopic port as part of the Ti2-LAPP configuration. Software-controlled mirrors direct TIRF illumination at any angle onto the objective back aperture, while NIS-Elements software enables precise definition and control of patterned illumination for both TIRF and photostimulation.

Key Features

Advanced TIRF, HILO, and photostimulation in one system

iLas2 is fully compatible with Nikon ECLIPSE Ti2-E inverted microscopes when configured through a Ti2-LAPP iluminator. Its mirror scanners provide 360°, arc, or point TIRF, reducing artifacts and supports fast ring illumination for improved image quality. A second scanner enables independent or simultaneous photostimulation, while true HILO imaging expands experimental depth. With a large effective field of view and built-in safety features, iLas integrates seamlessly for advanced multimodal imaging.

Ultimate TIRF uniformity with 360° illumination

By rotating the TIRF illumination beam at high speed, iLas2 can achieve ring-style 360° illumination. Conventional TIRF illumination, using a single illumination point for total internal reflection, produces undesired artifacts, interference fringes, and patterns as a result of the laser illumination.

By illuminating from multiple directions, these artifacts are removed, and the signal-to-noise ratio of the entire FOV is improved.

Precise control of illumination angle and direction

Illumination angle and direction can be precisely software controlled with iLas2 , independently for each illumination wavelength, to optimize penetration depth for any color. iLas2 is also capable of 360° HILO (highly inclined and laminated optical sheet), for oblique illumination for illumination at increased contrast.

Verify TIRF using Ti2’s aperture plane imaging

Users can safely and easily visualize TIRF laser illumination through a camera connected to the Ti2 photoport base, enabling simultaneous imaging of both the specimen plane and the objective back aperture. Total internal reflection can be verified at the aperture plane, while illumination calibration and focus are easily observed.

Stable illumination and large FOV over time with iLAS2 and Ti2-PFS

The iLas2 illuminator combines advanced laser scanning with galvanometer-controlled mirrors to deliver stable, uniform excitation across the entire field of view. By enabling 360°, arc, or point TIRF as well as HILO modes, iLas2 minimizes interference and artifacts while maintaining exceptional image quality. Paired with Ti2-PFS, researchers gain long-term stability for high-resolution live-cell and large-area imaging.

15 minute timelapse dual channel TIRF with 18mm TIRF FOV, maintaining focus position with PFS, using Apo 60X 1.49NA TIRF objective lens

Photostimulation: independently or in parallel with TIRF imaging

A second independent input light path to the iLas2 focuses a near diffraction-limited spot on the image plane, and allows rapid raster scanning of any complex shape for photostimulation. Illumination power and dwell time are independently controllable.

Photostimulation can be performed under any imaging modality, including widefield, confocal imaging, and TIRF.