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Long-term, high-resolution time-lapse imaging of axon self-assembly into motor nerve organoids in a microdevice

November 2023

Motor nerves are a collection of many axons assembled and aligned in one direction from the spinal cord to muscles. The fasciculation of axons provides them guidance, strength, and a healthy microenvironment. Therefore, it is of great significance to develop an in vitro model that mimics in vivo motor neuron properties and reproduces its structure.
Professor Yoshiho Ikeuchi et al., Institute of Industrial Science, The University of Tokyo, aims to reproduce the physiological environment of the human body using a microfluidic device.

This application note introduces an example of the spontaneous formation of motor nerve organoids in a microchip embedded in a 96-well plate, captured using a confocal microscope

Keywords: microdevices, motor nerve organoids, long-term time-lapse imaging, confocal.