Objectives for Multiphoton Microscopy
Bright images, high quality
Nikon offers a full lineup of high NA objectives for multiphoton imaging that provide chromatic aberration correction up to the near-infrared range. These objectives are optimized for deep imaging and provide bright images over the entire field of view.
Nano Crystal Coat for superior transmissivity
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/99331/AX_nano-coating_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/99331/AX_nano-coating_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
Nikon’s exclusive Nano Crystal Coat is an antireflective coating consisting of ultrafine crystalline particles. This forms a coarse structure that enables lower refractive indices, facilitating the passage of light through the lens rather than reflecting it, thus providing superior light transmission.
Water immersion objectives
The water immersion range of objectives provides the highest NA, high flexibility, and compatibility with upright, inverted, multiphoton or confocal instruments. The CFI75 models are dedicated to upright microscopes and imaging at greater depths (2~3 mm).
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230422/AXRMP_CFI75-Apochromat-LWD-20XC-W_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230422/AXRMP_CFI75-Apochromat-LWD-20XC-W_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI75 Apochromat LWD 20XC W
- WD (working distance): 2.80 mm
- NA: 1.00
- FOV: 22
- High-end MP lens
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230423/AXRMP_CFI75-Apochromat-20XC-W-1300_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230423/AXRMP_CFI75-Apochromat-20XC-W-1300_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI75 Apochromat 25XC W 1300
- WD: 2.00 mm
- NA: 1.10
- Chromatic aberration correction in the near-infrared range
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230433/AXRMP_CFI-Apochromat-Lambda-S-40XC-WI_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230433/AXRMP_CFI-Apochromat-Lambda-S-40XC-WI_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Apochromat Lambda S 40XC WI
- WD: 0.18 mm
- NA: 1.25, highest among water immersion objectives
- Optimal for confocal imaging
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230434/AXRMP_CFI-Apochromat-LWD-Lambda-S-20XC-WI_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230434/AXRMP_CFI-Apochromat-LWD-Lambda-S-20XC-WI_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Apochromat LWD Lambda S 20XC WI
- WD: 0.95 mm
- NA: 0.95
- High image quality and brightness
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230610/AXRMP_CFI75-LWD-16X-W_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230610/AXRMP_CFI75-LWD-16X-W_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI75 LWD 16X W
- WD: 3.00 mm
- NA: 0.80
- High transmittance in the near infrared range
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230611/AXRMP_CFI-Apochromat-NIR-40X-W_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230611/AXRMP_CFI-Apochromat-NIR-40X-W_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Apochromat NIR 40X W
- WD: 3.50 mm
- NA: 0.80
- Suitable for IR-DIC observation
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230607/AXRMP_CFI-Apochromat-NIR-60X-W_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230607/AXRMP_CFI-Apochromat-NIR-60X-W_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Apochromat NIR 60X W
- WD: 2.80 mm
- NA: 1.00
- Suitable for IR-DIC observation
Silicone immersion objectives
Silicone oil closely matches the refractive index of live cells, thereby minimizing spherical aberration and providing brighter, higher-resolution images in deep imaging.
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230609/AXRMP_CFI-Plan-Apochromat-Lambda-S-25XC-Sil_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230609/AXRMP_CFI-Plan-Apochromat-Lambda-S-25XC-Sil_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Plan Apochromat Lambda S 25XC Sil
- WD: 0.55 mm
- NA: 1.05
- FOV: 22
- High resolution with high NA
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230608/AXRMP_CFI-Plan-Apochromat-Lambda-S-40XC-Sil_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230608/AXRMP_CFI-Plan-Apochromat-Lambda-S-40XC-Sil_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Plan Apochromat Lambda S 40XC Sil
- WD: 0.30 mm
- NA: 1.25
- FOV: 22
- High resolution with high NA
Glycerol objectives
The glycerol range of objectives is dedicated to extreme depth (5~8 mm) observation of cleared samples. The major benefit of these lenses is their ability to correct for changes in refractive indices and compensate for aberrations that often occur in large sample imaging.
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230558/AXRMP_-CFI-Plan-Apochromat-10XC-Glyc_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230558/AXRMP_-CFI-Plan-Apochromat-10XC-Glyc_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
CFI Plan Apochromat 10XC Glyc
- WD: 5.50 mm (upright)/2.00 mm (inverted)
- NA: 0.50
- Wide refractive index correction range (1.33 to 1.51)
- Supports flexible sample preparation
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230559/AXRMP_CFI90-20XC-Glyc_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230559/AXRMP_CFI90-20XC-Glyc_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
CFI90 20XC Glyc
- WD: extremely long, 8.20 mm
- NA: 1.00
- Optimized for imaging of thick samples
Multi-immersion objectives
A multi-immersion objective can be used for oil immersion, glycerin immersion, and water immersion.
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230647/AXRMP_CFI-Plan-Fluor-20XC-MI_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230647/AXRMP_CFI-Plan-Fluor-20XC-MI_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Plan Fluor 20XC MI
- WD: 0.51-0.35 mm (oil), 0.51-0.34 mm (glycerin), 0.49-0.33 mm (water)
- NA: 0.75
- High UV wavelength transmittance
Dry objectives
This dry objective achieves uniform brightness up to the edge of the image, chromatic aberration correction from 405 nm to 850 nm, and high resolution. It provides wide area acquisition and high throughput.
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230652/AXRMP_CFI-Plan-Apochromat-Lambda-D-10X_3cb956f6342b0a39934cff4c79e64780.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AXR-MP/230652/AXRMP_CFI-Plan-Apochromat-Lambda-D-10X_77527181e037428e35eeb6eb6d9e348b.jpg 2x)
CFI Plan Apochromat Lambda D 10X
- WD: 4.00 mm
- NA: 0.45
- Supports low-magnification wide-area observation