Low Phototoxicity Long-Term Live Cell Apoptosis Assay Using Label-free Live Cell Imaging
Defective apoptosis is implicated in various diseases such as cancer, neurodegenerative diseases and autoimmune diseases.
Drug efficacy tests using apoptosis as an index are widely used in drug screening.
This application note introduces a long-term live cell apoptosis assay with low phototoxicity utilizing Volume Contrast images.
Total cell counts were measured using Volume Contrast images and apoptotic cells and dead cells were detected by fluorescent probes of Caspase-3/7 and EthD-1.