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Center of Excellence

University of Massachusetts Amherst

UMass Amherst’s Nikon Center of Excellence was established as part of a broad initiative to provide new and advanced research infrastructure to the local academic and non-academic community.  The mission of our NCoE is to provide trainees with in-depth knowledge of advanced microscopy techniques and training for the academic and biotechnology workforce.  Additionally, we provide opportunities to connect with industry professionals from Nikon and other partners.

Contact

CofE Director

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Address

Institute for Applied Life Sciences
University of Massachusetts Amherst
Massachusetts


Systems Available

A1R-SIMe: Nikon A1 Resonant Scanning Confocal with Structured Illumination Super-Resolution

This microscope is very versatile and can be used for live or fixed samples. The resonant scanner allows for very fast acquisitions and the GaAsP detectors are extremely sensitive. The SIM side of the microscope is extremely easy to use with no special sample preparation required for super-resolution imaging.

View Components

  • Objectives: 10x, 20x, 40x, 60x, 100x, 60x dry
  • Laser lines: 405, 488, 561, 640nm for confocal; 488, 561, 640nm for SIM
  • Detectors: 5 detectors: 1 transmitted light, 2 high-sensitivity PMTs, and 2 GaAsP detectors; sCMOS for SIM

SD: Nikon with Yokogawa Spinning Disk Confocal and Orthogonal Stimulation

This microscope is great for live cells as it is a low-light technique. With four laser lines and an additional mini-scanner for PA/FRAP/etc., we can easily image dynamic movements in live cells, stimulating/bleaching in real time.

View Components

  • Objectives: 20x, 40x water immersion, 40x oil immersion, 60x, 100x
  • Laser lines: 405, 488, 561, 640nm for imaging and 405, 488, 561, 640nm for photoactivation/bleaching/etc.
  • Detectors: Andor EMCCD camera
  • Stage: Piezo

LCMD: Nikon with Arcturus Laser Capture Micro-Dissection

This microscope is really a cellular robot. You can find cells or regions using brightfield or fluorescence that you are interested on tissue slices and draw a line around them, cut them out, move them to a cap and then process the cap for downstream experiments (sequencing, proteomics, etc.).

  • Objectives: 4x, 10x, 40x
  • Laser lines: UV, IR
  • Detectors: Color camera

A1R: Nikon A1 Resonant Scanning Confocal with TIRF Module

This microscope is highly versatile and can be used for live or fixed samples. The resonant scanner allows for very fast acquisitions and the GaAsP detectors are extremely sensitive. This microscope has 6 lasers and the full gamut of objectives and software modules.

View Components

  • Objectives: 10x, 20x, 40x, 60x, 100x, 60x TIRF, 100x TIRF
  • Laser lines: 405, 435, 488, 514, 561, 640nm
  • Detectors: 5 detectors: 1 transmitted light, 2 high-sensitivity PMTs, and 2 GaAsP detectors
  • Camera: Andor Xyla

A1SP: Nikon A1 Spectral Detector Confocal with FLIM Module

This microscope is great for fixed samples and is especially useful when experimenters may have overlapping emissions from fluorophores or autofluorescence. The 32-channel spectral detector can be implemented with the click of a button and allows for 32 x 2.5 – 10nm bins of fluorescence identification.

View Components

  • Objectives: 10x, 20x, 40x, 60x, 100x
  • Laser lines: 405, 488, 561, 640nm
  • Detectors: 5 detectors: 1 transmitted light, two high-sensitivity PMTs, and 2 GaAsP detectors

A1MP: Nikon A1 Resonant Scanning Multi-Photon Confocal

This microscope is an upright, manual microscope that is suited for in vivo, intravital imaging as well as imaging in and through thick tissues and samples. It uses a tunable infrared pulsed laser to excite fluorophores at the focal volume and features a resonance scanner that can image very quickly along with a fast moving piezo nosepiece. We also have visible lasers for standard upright confocal microscopy.

View Components

  • Objectives: 25x extremely long working distance (upright)
  • Laser lines: 760-1040nm
  • Detectors: 1 high-sensitivity PMT, 3 GaAsP detectors

HCA: Nikon with High Content Analysis

This microscope is truly amazing for its ability to collect and automatically analyze data from live or fixed samples. The intuitive and adaptive software can be programed to count cells, monitor growth, take high-resolution pictures when a certain feature is found, scan slides, scan multi-well plates, etc. A robot can even load your multi-well plates. It has two imaging paths; wide-field and spinning disk. When it is done, you can have the microscope send you a text message that contains any key variables that you need to know right away.

View Components

  • Objectives: All “air” objectives; 10x, 20x, 20xELWD, 40x, 40xELWD, 60x
  • Excitation colors: 395, 415, 445, 488, 515, 540, 590, 640
  • Detectors: Widefield with an Andor Zyla sCMOS camera and also a Crest spinning disk confocal with a ProEM camera.

N-STORM: Nikon STORM

This microscope makes doing 3D STORM imaging straightforward. This has STORM-4.0 which includes a cylindrical lens to provide z-information on your molecules of interest. This also has the option to change not only the TIRF angle with the click of a button, but also the direction of the laser entering the back aperture of the objective.

View Components

  • Objectives: 20x, 60x TIRF, 100 TIRF (HP)
  • Laser lines: 405, 488, 561, 640nm
  • Detectors: Hamamatsu ORCA-Flash4.0