Applications

2D Imaging

We provide imaging services for a variety of 2D model systems. These services include a broad portfolio of assays, live and fixed cell imaging on slides coverslips, dishes and multi-well plates, epifluorescence or confocal imaging modalities, and intelligent image acquisition and AI-supported analysis. Our broad portfolio of assays captures our strength, and are highly customizable to client-specific needs.

3D Imaging

We provide imaging services for a variety of 3D model systems. These services include a broad portfolio of assays, live and fixed cell imaging, time-lapse imaging, whole organism imaging, epifluorescence or confocal imaging modalities, intelligent image acquisition, and AI-supported analysis. Our broad portfolio of assays captures our strength, and are highly customizable to client-specific needs.

Whole Slide Scanning

We provide whole slide imaging services, brightfield or fluorescence, of fixed specimens for histology, pathology, gene expression, and spatial biology studies.

2D or 3D Toxicology, Uptake, and Viability Studies

This versatile service module provides customized bioimaging services for the purposes of studying cellular health and activity in 2D or 3D cultures. Possible experiments range from basic live/dead assays to organelle characterization in 3D spheroids.

mRNA Quantification (smFISH) for Gene Expression Analysis

Single molecule fluorescence in situ hybridization (smFISH), known for its ability to quantify individual RNA molecules, is widely used to characterize spatio-temporal patterns of gene expression in single cells. At NBIL we harness this technique to provide RNA quantification services for research applications such as gene expression analysis in response to drug candidates, spatial biology studies, and more.

Organ-on-chip Applications

Organ-on-chip models enable the study of multicellular and tissue-level behavior in cells cultured on a scaffold that promotes organ-like conditions and morphology. These platforms are useful for providing a more physiological context to basic research and drug discovery experiments.

We collaborate with manufacturers such as Nortis, TissUse, and Mimetas. Customer-provided devices are welcome.

Custom Image Processing and Analysis

Image processing has a wide variety of uses and is an integral of most microscopy experiments, from clarifying low-signal images to preparing images for robust segmentation and quantification. Custom analysis workflows are used for applications from gathering single-cell statistics to training AI networks for detection of customer-defined features.

Transendothelial Migration

Transendothelial migration (TEM) describes the process of immune cell extravasation. Our immune cells protect our body from foreign substances, infections, and cancer. To achieve this, immune cells travel in the bloodstream to reach sites of inflammation. Such sites provide inflammatory signals which are relayed to the vascular lumen by endothelial cells lining the vascular lumen. The constant stream of immune cells passing by gets activated and allows them to enter the tissue to clear the infection. Extravasation at and the invasion of tumor tissue by immune cells for example stands central to modern therapies like adoptive transfer of therapeutic T cells or NK cells.

Histological Image Segmentation

Histological image analysis is a crucial aspect of medical research and diagnostics. It involves the microscopic examination of tissue samples that have been stained to highlight different cellular components and structures. This analysis allows pathologists to diagnose diseases, understand cellular interactions, and conduct detailed anatomical studies. The accuracy of histological analysis is vital, as it directly impacts clinical decisions and the understanding of complex biological processes.

Research Areas

  • Drug Discovery
  • Basic Research
  • Gene Expression
  • Digital Pathology
  • Immuno-oncology
  • Spatial Biology
  • Histology
  • Toxicology
  • Cell Biology
  • 3D Culture Systems

Imaging Capabilities

  • 4 – 100X Objectives with water, silicone, and oil immersion options
  • Incubation
  • Fluorescence imaging
  • Brightfield contrast (Phase, DIC NAMC)
  • Widefield
  • Confocal
  • Super-resolution confocal (NSPARC)
  • Spinning disk
  • Super-resolution Spinning disk (SoRA)
  • STORM
  • FLIM
  • FCS
  • TIRF
  • FRET
  • FRAP
  • Automated imaging
  • Image analysis

NBIL Study Topics

  • Live-cell imaging
  • Size and morphology analysis
  • Cell counting
  • Cytotoxicity
  • Transfection efficiency
  • Fluorescence intensity measurement
  • Apoptosis
  • DNA damage
  • Nuclear translocation
  • Autophagy
  • Endocytosis
  • Phagocytosis
  • Mitochondrial analysis
  • Chromogenic staining
  • Immunofluorescence

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