
NIR Imaging Option
Incorporating the NIR Imaging Option enables near-infrared excitation in addition to conventional visible-light excitation. It allows the acquisition of more channels in multicolor fluorescence imaging, contributing to the understanding of complex structures in living specimens.

Merged Image
Hauptmerkmale
Bright, high-definition deep imaging
Near-infrared light has a high penetration depth into tissue and is less affected by light absorption and scattering. Acquiring near-infrared light with a high S/N ratio therefore enables high-definition observation of deep structures within living specimens. In addition, near-infrared light induces less sample autofluorescence which improves imaging clarity, and its low phototoxicity makes it effective for imaging living cells.

DAPI

Alexa Fluor® 488

Alexa Fluor® 568

Alexa Fluor® 750
Multi-stained specimen of HeLa cells acquired with visible and NIR light using the NIR imaging option.
Nucleus (DAPI), Actin (Alexa Fluor® 488), Tubulin (Alexa Fluor® 568), Mitochondria (Alexa Fluor® 750)
High wavelength selection flexibility
NIR imaging extended to the 730 nm and 785 nm lines allows excitation of fluorophores over a wide wavelength range from violet to near-infrared, which is effective for reducing crosstalk between fluorescent channels. Excitation in the NIR allows acquisition of additional dyes and thereby more structural details of specimens in multichannel imaging.