Moffitt Cancer Center

Opening: Tuesday, March 4, 2025; From left to right: Jose Serrano-Velez, Ph.D. (Couch BioImaging Director), Ric Villani (Regional Sales Manager, Nikon Instruments), Suparna Narayan (Field Representative for Congresswoman Laurel Lee), Andrew Brumm, Ph.D. (Advanced Biosystems Manager, Nikon Instruments), Greg Sawyer, Ph.D. (Chair of the Bioengineering Department at Moffitt), John Cleveland, Ph.D. (Executive Vice President, Center Director and Chief Scientific Officer at Moffitt), Ted Couch (National Board of Advisors at Moffitt), Tim Adams (Chair, Institute Board of Directors at Moffitt), Patrick Hwu, M.D. (President and CEO at Moffitt)

Contact

Couch BioImaging Director

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Address

Moffitt Cancer Center
12902 USF Magnolia Drive
SRB 4th Floor, Suite 24045
Tampa, FL 33612

Systems Available

AX R MP Multiphoton

The AX R MP is Nikon’s newest large format multiphoton microscope, ideal for deep imaging. The highly improved 2K resonant scanner allows users to acquire high resolution images of fast in vivo events. It is combined with visible wavelengths and tunable emission detectors for maximum capabilities.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
AX R MP hybrid scanner with 2K resonant
Coherent Discovery NX IR Laser
4-laser line visible unit (405/488/561/640nm)
GaAsP non-descanned (NDD) detectors
DUX-VB4 4ch confocal fixed/tunable NIR detector
WID automatic water dispenser
NIS-Elements Software

AX R MP Multiphoton

Nikon’s latest large-format multiphoton microscope, the AX R MP, is designed for deep imaging. The highly improved 2K resonant scanner allow users to acquire high resolution images of fast in vivo events.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
AX R MP hybrid scanner with 2K resonant
Coherent Discovery NX IR Laser
GaAsP non-descanned (NDD) detectors
WID automatic water dispenser
NIS-Elements Software

AX R with NSPARC Confocal

The AX R is a recently developed iteration of Nikon’s flagship confocal system, and is capable of 2k resonant scanning in an expanded 25 mm FOV for fast, gentle imaging of sensitive samples. In addition, this system features Nikon’s new NSPARC Image Scanning detector, capable of deep, super-resolution imaging using a highly sensitive APD detector array.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
AX R hybrid scanner with 2K resonant
4-laser line unit (405/488/561/640nm)
DUX-VB4 4ch confocal fixed/tunable detector
NSPARC spatial array detector
NIS-Elements Software

AX R Confocal

The AX R is Nikon’s latest generation laser scanning confocal system with a 25 mm field of view, capable of 2K resonant scanning capabilities. The highly sensitive hybrid detectors allow for fast and gentle imaging of sensitive samples while significantly improving the time of data acquisition of fixed specimens.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
AX R hybrid scanner with 2K resonant
4-laser line unit (405/488/561/640nm)
DUX-VB4 4ch confocal fixed/tunable detector
NIS-Elements Software

A1R HD25 Confocal

The A1R HD25 laser scanning confocal system is a versatile imaging system integrated with a BioTribometer that allows measurement of friction and adhesion of cellular surfaces during imaging.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
A1R HD25 hybrid scanner with 1K resonant
6-laser line unit (405/445/488/514/561/640nm)
DUG 4ch confocal fixed/tunable detector
NIS-Elements Software

A1R HD25 Confocal

The A1RHD25 laser scanning confocal system is a versatile imaging system integrated with an Atomic Force Microscope (AFM) for topographgical imaging.

Components

ECLIPSE Ti2-E inverted microscope with Perfect Focus System
A1R HD25 hybrid scanner with 1K resonant
5-laser line unit (405/488/561/640/730nm)
DUG 4ch confocal fixed/tunable detector
NIS-Elements Software

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