Application Notes
![](https://downloads.microscope.healthcare.nikon.com/production/imager/coverimages/Application-Notes/3277/N-SIM-for-Quantitative-Ultra-Structural-Analyses-of-the-Nuclear-Lamina_8f52416f9afcc73a2e17f8c24fa98668.jpg)
N-SIM for Quantitative Ultra-Structural Analyses of the Nuclear Lamina
October 2016
Super-resolution Structured Illumination Microscopy (SIM), available from Nikon via the N-SIM S and N-SIM E systems, allows for the observation of details inaccessible to traditional microscopes, such as confocal and widefield. In this application note we see how the N-SIM system enables quantitative multi-color evaluation of the distribution of different nuclear lamin proteins and the structures they form.