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NIR Imaging Option
Incorporating the NIR Imaging Option enables near-infrared excitation in addition to conventional visible-light excitation. It allows the acquisition of more channels in multicolor fluorescence imaging, contributing to the understanding of complex structures in living specimens.
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/285512/AX-NIR_Bright-high-definition-deep-imaging-0213-rambda-D-60x-2K-Gal3-1AUat561-Z10-snapshots_ec7d1033075031a0254a7350f4e87eb4.jpg)
Merged Image
Características principales
Bright, high-definition deep imaging
Near-infrared light has a high penetration depth into tissue and is less affected by light absorption and scattering. Acquiring near-infrared light with a high S/N ratio therefore enables high-definition observation of deep structures within living specimens. In addition, near-infrared light induces less sample autofluorescence which improves imaging clarity, and its low phototoxicity makes it effective for imaging living cells.
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285850/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_DAPI_bf846fc1a489c5e237a674bd79051942.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285850/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_DAPI_e8d850819e27cea92f38321455d96b39.jpg 2x)
DAPI
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285851/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_AF488_bf846fc1a489c5e237a674bd79051942.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285851/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_AF488_e8d850819e27cea92f38321455d96b39.jpg 2x)
Alexa Fluor® 488
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285848/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_AF568_bf846fc1a489c5e237a674bd79051942.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285848/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_AF568_e8d850819e27cea92f38321455d96b39.jpg 2x)
Alexa Fluor® 568
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285849/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_AF750_bf846fc1a489c5e237a674bd79051942.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/AX/NIR/285849/AX-NIR_0213-rambda-D-60x-2K-Gal3-1AUat561-Z10_AF750_e8d850819e27cea92f38321455d96b39.jpg 2x)
Alexa Fluor® 750
Multi-stained specimen of HeLa cells acquired with visible and NIR light using the NIR imaging option.
Nucleus (DAPI), Actin (Alexa Fluor® 488), Tubulin (Alexa Fluor® 568), Mitochondria (Alexa Fluor® 750)
High wavelength selection flexibility
NIR imaging extended to the 730 nm and 785 nm lines allows excitation of fluorophores over a wide wavelength range from violet to near-infrared, which is effective for reducing crosstalk between fluorescent channels. Excitation in the NIR allows acquisition of additional dyes and thereby more structural details of specimens in multichannel imaging.