King’s College London

AX R Confocal with ECLIPSE Ti2-E Inverted Microscope

Nikon's AX R confocal features a 25 mm FOV, up to 8192 x 8192 pixel resolution, and is equipped with AI-based tools for simplifying acquisition and analysis. The AX R includes our high definition resonant scanner, which features a next-generation optical non-linear sampling digitizer, supporting imaging at high pixel densities of up to 2048 x 2048, and with superior image quality compared to other currently available resonant scanning systems.

ECLIPSE Ti2-E with N-STORM 5.0 TIRF

The Nikon N-STORM 5.0 system is built on the ECLIPSE Ti2 inverted microscope platform and is optimized for super-resolution imaging using Single Molecule Localization Microscopy (SMLM), including STORM and PALM techniques. The system features TIRF illumination for precise imaging near the coverslip and is equipped with either sCMOS or dual EMCCD cameras via a Cairn TwinCam splitter for multi-channel acquisition. A CoolLED p4000 provides stable and flexible epifluorescence illumination. This setup enables imaging at ~20 nm resolution, making it ideal for studying protein complex clustering, sub-organelle localization, focal adhesions, and molecular dynamics.

CSU-X1 Spinning Disk Confocal with ECLIPSE Ti2-E Inverted Microscope

The Yokogawa CSU-X1 spinning disk confocal combined with Nikon’s ECLIPSE Ti2-E inverted research microscope platform provides fast, high-resolution 4D imaging of living cells with minimal effects of photo-damage. The Ti2-E microscope is equipped with Perfect Focus (PFS) to enable long time-lapse imaging without focus drift. The system is also equipped with a fast, laser-based raster-scanning device (Miniscanner) for photobleaching and photostimulation.

ECLIPSE Ti2-E Inverted Microscope System with Dual Camera

The ECLIPSE Ti2 delivers an unparalleled 25mm field of view (FOV), allowing you to capture twice as much data with a single image. The fourth generation Perfect Focus System combined with a completely redesigned Z-drive provide a super-stable imaging platform with high-precision focusing for demanding applications. Built-in sensors and intelligent Assist Guide eliminate user error while the internal detector enables you to see the back-aperture for alignments. This system is equipped with dual Andor Neo sCMOS camera.

A1R Multiphoton with ECLIPSE Ni-E FN Upright Microscope

Nikon's A1R HD multiphoton microscope enables high speed, deep tissue imaging in live organisms with unprecedented sensitivity and clarity. The system features an ultra-high speed resonant scanner capable of 420 fps imaging, a galvanometric scanner for high-resolution imaging, high-sensitivity GaAsP Multi Detector in both episcopic and diascopic imaging paths to optimize signal detection and high N.A. objective lenses designed for high-resolution, deep tissue imaging.

ECLIPSE Ti-E with VT-iSIM

The Visitech's iSIM is an instant structured illumination microscopy (iSIM) system integrated with a Nikon ECLIPSE Ti-E inverted microscope. It offers increased lateral resolution (~120 nm) compared to conventional widefield or confocal systems, while maintaining high imaging speeds suitable for live-cell imaging.

The system supports multi-channel fluorescence acquisition and fast volumetric imaging, making it suitable for applications involving dynamic cellular processes. The Ti-E base provides motorized control, Nikon’s Perfect Focus System (PFS) for focus stability during time-lapse imaging, and compatibility with a wide range of objective lenses.

Image acquisition and hardware control are managed through Nikon’s NIS-Elements software. The system is suited for users requiring super-resolution imaging with relatively low phototoxicity and minimal sample preparation compared to other super-resolution methods.