2D or 3D Toxicology, Uptake, and Viability Studies

This versatile service module provides customized bioimaging services for the purposes of studying cellular health and activity in 2D or 3D cultures. Possible experiments range from basic live/dead assays to organelle characterization in 3D spheroids.

Toxicity Live
Toxicity DAPI
Toxicity Dead
Toxicity Overlay

Live-Dead Assay on Spheroids as Platform for Toxicology Studies for counting EtBR-stained nuclei vs. total nuclei

Challenges

Image quality is paramount for detecting subtle differences between cells, particularly in complex samples that may introduce optical aberrations. Additionally, it can be difficult to automatically identify and segment subcellular features for analysis. Finally, multitudes of data points are required to properly form strong conclusions, but acquiring vast amounts of high-quality imaging data typically requires time and expertise.

Solutions

Nikon’s diverse lineup of world-renowned objective lenses are designed to optimally image a huge variety of sample types. Acquiring clear images enables powerful, repeatable image analysis. The NIS-Elements software is equipped with a full toolbox of image processing, segmentation, and analysis options, including AI-driven functions for pre-defined and customizable feature identification. It is also possible to develop hardware routines to increase imaging throughput and complexity.

The NBIL is staffed with expert assay scientists & microscopists who will work with you to develop and execute customized image acquisition and analysis workflows to advance your research.

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