stage micrometer A microscope calibration slide with precisely spaced markings. Related MicroscopyU Article Eyepiece Reticle Calibration Linear measurements performed with a compound optical microscope generally cover a length range of approximately 25 millimeters down to 0.2 micrometer, upper and lower boundaries that are determined primarily by the maximum viewfield diameter and the microscope limit of resolution. This interactive tutorial explores calibration of various eyepiece reticles with a stage micrometer, and demonstrates how the reticle can then be employed to determine linear specimen dimensions.